Device for measuring the fluorescence of a medium

• US 8,872,133 B2
• Filed: 05/07/2010
• Issued: 10/28/2014
• Est. Priority Date: 05/07/2009
• Status: Active Grant

• Current Assignee: Krohne Optosens GmbH
• Original Assignee: Krohne Optosens GmbH
• Inventors: Schreiber, Marco, Fritsch, Thomas
• Primary Examiner(s): Porta, David
• Assistant Examiner(s): GUNBERG, EDWIN C
• Application Number:
  US12/775,855
• Publication Number:
  US 20100282982A1
• Time in Patent Office: 1,635 Days
• Field of Search: 250/459.1
• US Class Current: 250/459.1
• CPC Class Codes: G01N 2021/6491 Measuring fluorescence and transmission; Correcting inner filter effect
• CPC Class Codes: G01N 21/53 within a flowing fluid, e.g. smoke
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence
• CPC Class Codes: G01N 21/85 Investigating moving fluids or granular solids
• CPC Class Codes: G01N 2201/1211 for temperature

Abstract

A device for measuring the fluorescence of a medium having a radiation source, an emission-receiving element and an optical imaging element arranged on the sensor side of the optical imaging element, and a scattering-receiving element arranged on the sensor side of the optical imaging element and in which the radiation source, the imaging element and the emission-receiving element are aligned and configured relative to one another so that the medium present on the medium side of the imaging element can be illuminated by radiation from the radiation source, and the emission intensity of the medium radiation emitted by the medium based on fluorescence can be detected with the emission-receiving element. To provide a device for measuring the fluorescence of a medium which has an increased reliability in measuring the fluorescence, temperature compensation is performed relative to the temperature of the medium and/or at least one of the receiving elements.

Claims

1. A method for fluorescent spectroscopic in-line determination of the protein content of a medium containing milk, comprising the steps of:

illuminating the medium by radiation from a radiation source;

guiding a part of the radiation from said radiation source by a beam splitter to a reference receiving element that detects the source intensity of said radiation source;

detecting an emission intensity of a medium radiation emitted by the medium based on fluorescence with an emission-receiving element;

detecting a scattering intensity of the medium radiation scattered by the medium in a range of the absorption of the radiation of the radiation source caused by fluorescence with a scattering-receiving element;

measuring the temperature of the medium;

measuring the temperature of at least one of the receiving elements;

compensating the respective detection of radiation by the at least one of the receiving elements for an effect of the measured temperature of the least one of the receiving elements; and

converting the detected emission intensity, in combination with the detected scattering intensity, and in combination with a compensation for an effect of the measured temperature of the medium on the fluorescence of the medium, into a concentration value of fluorescent parts of the medium,

wherein said radiation source, said beam splitter, and said reference receiving element are commonly arranged on a holding device such that a main receiving direction of said reference receiving element and a main direction of the radiation of said radiation source are at a 90°

angle to one another, and

wherein the main direction of radiation of the radiation source is parallel to a main receiving direction of said emission-receiving element and to a main receiving direction of said scattering-receiving element.

2. The method according to claim 1, wherein:

the measuring the temperature of the at least one of the receiving elements comprises measuring the temperature of two of the receiving elements; and

the compensating comprises compensating the detection of the radiation by the two receiving elements for an effect of the respective measured temperature of the two of the receiving elements.

3. The method according to claim 1, wherein the fluorescence of tryptophan is used for the determination of the protein content of the medium containing milk.

4. The method according to claim 1, wherein the radiation from the radiation source is filtered by an optical filter which is maximally permeable for the frequency range of the radiation with which the fluorescence of the medium is excitable.

5. The method according to claim 1, wherein the medium radiation is filtered before being detected by the emission-receiving element by an optical emission filter which is maximally permeable for the frequency range in which the medium radiation based on fluorescence of the medium lies.

6. The method according to claim 1, wherein the medium radiation is filtered before being detected by the scattering-receiving element by an optical scattering filter which is maximally permeable for the frequency range in which the medium has its absorption maximum caused by fluorescence of the medium.