Method and device for optical detection of substances in a liquid or gaseous medium

• US 8,877,129 B2
• Filed: 07/16/2008
• Issued: 11/04/2014
• Est. Priority Date: 07/16/2007
• Status: Active Grant

• Current Assignee: Fraunhofer Gesellschaft Zur Foerderung Der Angewandten Forsching E.V.
• Original Assignee: Fraunhofer Gesellschaft Zur Foerderung Der Angewandten Forsching E.V.
• Inventors: Brandenburg, Albrecht
• Primary Examiner(s): Mui, Christine T
• Application Number:
  US12/174,294
• Publication Number:
  US 20090069199A1
• Time in Patent Office: 2,302 Days
• Field of Search: 422/82.11
• US Class Current: 422/82.11
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/648 using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 33/544 the carrier being organic
• CPC Class Codes: G01N 33/552 Glass or silica

Abstract

The invention relates to a device for optical detection of substances in a liquid or gaseous medium, with a substrate with molecules for detecting the substances that are to be detected, wherein these molecules are immobilized at a surface of the substrate or in the substrate and wherein the substances that are to be detected can essentially be selectively bound to these molecules, wherein light waves can be coupled into the substrate and can be guided through this, and wherein the substrate is a foil element made of a transparent material in which a coupling structure for coupling the light waves is integrally formed and in which the coupled light waves can be guided.

Claims

1. A device for optical detection of substances in a liquid or gaseous medium, comprising:

a substrate with fluorescent molecules immobilized thereto for detecting substances that are selectively bindable to said fluorescent molecules;

said substrate comprising a polymeric foil element comprising an optically transparent plastic material having a thickness of from 10 to 1000 microns;

said foil element having at least one light wave coupling structure capable of guiding coupled light waves, the light wave coupling structure including a prism; and

said coupling structure being integrally formed with the foil element at a surface of the foil element, said coupling structure being located at an area of the foil element adapted to be brought into contact with the substances to be detected, wherein the coupling structure is a refractive optical element;

wherein the prism is provided for simultaneously with the formation of the film in a mold made for coining, rolling or injection molding, and the prism is provided for in one step with the making of the device.

4. The device according to claim 1, wherein the immobilized molecules form a readout field with a multiplicity of measurement points for spatially-resolved detection of the selectively bindable substances.

5. The device according to claim 4, wherein the foil element has an homogenizing area in a beam path downstream of the coupling structure and upstream of the readout field.

6. The device according to claim 4, further including a light wave homogenizing element in a beam path downstream of the coupling structure and upstream of the measurement points.

9. The device according to claim 1, wherein said foil element has a thickness of up to 130 μ

m.

10. The device according to claim 9, wherein the foil element is includes an outer area that frames an inner area, the outer area having a thickness greater than a thickness of the inner area.

19. The device according to claim 1, wherein optically transparent plastic material comprises an organic polymer or copolymer.

21. The device according to claim 1, wherein said molecules are immobilized at a surface of said substrate.

22. The device according to claim 1, wherein said molecules are immobilized in said substrate.

23. The device according to claim 6, wherein said light wave homogenizing element is a diffraction grating.

25. The device according to claim 10, wherein said outer area provides increased mechanical stability to the foil element.

2. A device for optical detection of substances in a liquid or gaseous medium, comprising:

a substrate with fluorescent molecules immobilized thereto for detecting substances that are selectively bindable to said fluorescent molecules;

said substrate comprising a polymeric foil element comprising an optically transparent plastic material having a thickness of 10 to 1000 microns;

said foil element having at least one light wave coupling structure capable of guiding coupled light waves, the light wave coupling structure including a prism, the coupling structure being integrally formed with the foil element at a surface of the foil element, the coupling structure located at an area of the foil element adapted to be brought into contact with the substance to be detected, wherein the coupling structure is a refractive optical element; and

a flow cell for the medium to be examined, said flow cell forming a measurement area with a surface of the foil element, wherein the coupling structure is in the measurement area;

wherein the prism is provided for simultaneously with the formation of the film in a mold made for coining, rolling or injection molding, and the prism is provided for in one step with the making of the device.

3. The device according to claim 2, wherein the immobilized molecules are probes comprising biological or biochemical molecules selected from the group consisting of antibody molecules, DNA molecules, DNA single strands, RNA molecules, RNA single strands, and mixtures thereof.

7. The device according to claim 2, wherein the coupling structure has a curvature.

8. The device according to claim 2, wherein the coupling structure is a diffractive optical element.

11. The device according to claim 2, wherein the foil element is integrated into a polymer cartridge, said polymer cartridge having an injection port for the medium that is to be examined, reagent containers, fluid canals for transporting the medium and reagents, and devices for moving the medium and the reagents.

18. The device according to claim 3, further comprising focusing optics with which optical characteristics of substances or labels bound to the probes can be measured.

20. The device according to claim 18, wherein the foil element comprises a hot stamped glass.

24. The device according to claim 2, wherein the refractive optical element is a prism or a grating or a structure with a trapezoidal or rectangular cross-section.

28. The device according to claim 18, wherein said optical characteristics comprise fluorescence or color.

29. The device according to claim 2, wherein said foil element has a thickness of up to 130 microns.

12. A device for optical detection of substances in a liquid or gaseous medium, comprising:

a substrate with fluorescent molecules immobilized thereto for detecting substances that are selectively bindable to said fluorescent molecules;

said substrate comprising a polymeric foil element comprising an optically transparent plastic material having a thickness of 10 to 1000 microns in which a coupling structure capable of guiding light waves is integrally formed at a surface of the foil element, the light wave coupling structure including a prism, the coupling structure being located at an area of the foil element adapted to be brought into contact with the substances to be detected, wherein the coupling structure is a refractive optical element; and

said coupling structure comprising excitation optics for producing light waves for exciting a label used for optical detection, or for producing a color change at the foil element;

wherein the prism is provided for simultaneously with the formation of the film in a mold made for coining, rolling or injection molding, and the prism is provided for in one step with the making of the device.

13. The device according to claim 12, further including a device for changing a relative position of excitation optics to the coupling structure, wherein a coupling angle can be selected to roughly correspond to a total reflection angle to provide a refractive index of the medium adjacent to the foil element.

14. The device according to claim 13, further including a decoupling structure in the foil element, wherein the decoupling structure is for decoupling coupled light waves and is formed at the surface of the foil element during formation step of the foil element.

15. The device according to claim 14, wherein the decoupling structure is a refractive optical element or a diffractive optical element.

16. The device according to claim 15, wherein a detector is in a beam path downstream of the decoupling structure, wherein the light waves decoupled from the decoupling structure can be detected by this detector for optimizing the beam position or for determining the scattered light of the coupled light waves.

17. The device according to claim 15, wherein a detector for detection of an intensity of the light waves is provided at the decoupling structure for determining absorption of the light waves guided in the foil element and the associated change of energy state of the light waves.

26. The device according to claim 12, wherein said label is a fluorescent dye.

27. The device according to claim 12, wherein said foil element has a thickness of about 100 microns.