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Culture substrate for human pluripotent stem cells and use thereof

  • US 8,877,493 B2
  • Filed: 10/07/2010
  • Issued: 11/04/2014
  • Est. Priority Date: 10/08/2009
  • Status: Active Grant
First Claim
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1. A culture method of human pluripotent stem cells dissociated into single cells in feeder-free culture conditions, comprising the steps of:

  • dissociating human pluripotent stem cells into single cells, andculturing the human pluripotent stem cells dissociated into single cells using a culture substrate, the culture substrate being coated with human laminin β





    1 E8 fragment or human laminin α





    2 E8 fragment.

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