Method of differentiating mammalian progenitor cells into insulin producing pancreatic islet cells
First Claim
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1. A method for differentiating hepatic progenitor cells into insulin-producing pancreatic cells, comprising:
- (a) incubating hepatic progenitor cells expressing albumin and CK19 in serum-free pre-induction media comprising sodium butyrate; and
, subsequently,(b) incubating the hepatic progenitor cells in induction media comprising knock-out replacement serum;
for a time sufficient to differentiate the hepatic progenitor cells into insulin-producing pancreatic cells.
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Abstract
The invention relates to methods for differentiating progenitor cells into insulin producing pancreatic islet cells and compositions and methods for using such cells.
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Citations
7 Claims
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1. A method for differentiating hepatic progenitor cells into insulin-producing pancreatic cells, comprising:
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(a) incubating hepatic progenitor cells expressing albumin and CK19 in serum-free pre-induction media comprising sodium butyrate; and
, subsequently,(b) incubating the hepatic progenitor cells in induction media comprising knock-out replacement serum; for a time sufficient to differentiate the hepatic progenitor cells into insulin-producing pancreatic cells. - View Dependent Claims (2, 3, 7)
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4. A method for differentiating hepatic progenitor cells into insulin-producing pancreatic cells, comprising:
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(a) incubating hepatic progenitor cells expressing albumin and CK19 in serum-free pre-induction media comprising a histone deacetylase inhibitor (HDACI) selected from the group consisting of phenyl butyrate, retinoic acid, valproic acid, APHA Compound 8, Apicidin, (−
)-Depudecin, Scriptaid, Sirtinol, and Trichostatin; and
, subsequently,(b) incubating the hepatic progenitor cells in induction media comprising knock-out replacement serum; for a time sufficient to differentiate the hepatic progenitor cells into insulin-producing pancreatic cells. - View Dependent Claims (5, 6)
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Specification