Antimicrobially active compositions

• US 8,877,818 B2
• Filed: 05/21/2007
• Issued: 11/04/2014
• Est. Priority Date: 04/07/2005
• Status: Active Grant

• Current Assignee: CDH Bioscience Incorporated
• Original Assignee: CHD Bioscience, Inc., XY, LLC (Inguran LLC)
• Inventors: Neas, Edwin Dean, Kuiken, Jerald Edward, Schenk, John Louis, Gilligan, Thomas Boyd
• Primary Examiner(s): Blanchard, David J
• Assistant Examiner(s): FRAZIER, BARBARA S
• Application Number:
  US11/804,879
• Publication Number:
  US 20080090917A1
• Time in Patent Office: 2,724 Days
• Field of Search: None
• US Class Current: 514/675
• CPC Class Codes: A01N 2300/00 Combinations or mixtures of active ingredients covered by classes A01N27/00 - A01N65/48 with other active or formulation relevant ingredients, e.g. specific carrier materials or surfactants, covered by classes A01N25/00 - A01N65/48
• CPC Class Codes: A01N 25/00 Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application , e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
• CPC Class Codes: A01N 25/02 containing liquids as carriers, diluents or solvents
• CPC Class Codes: A01N 31/02 Acyclic compounds
• CPC Class Codes: A01N 31/06 Oxygen or sulfur directly attached to a cycloaliphatic ring system
• CPC Class Codes: A01N 35/02 containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
• CPC Class Codes: A01N 35/06 containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
• CPC Class Codes: A61L 2/18 Liquid substances or solutions comprising solids or dissolved gases

Abstract

Compositions (3) and methods of using such compositions (3) to condition, clean, or disinfect the flow path of a conduit (8) of a microfluidic devices (16), such as flow cytometers or liquid chromatographs.

Claims

1. A method of reducing a number of viable microorganisms in a flow path of a microfluidic device, comprising:

a) flowing an amount of an antimicrobial composition within said flow path, wherein said antimicrobial composition includes;

i. an antimicrobially active agent of an alcohol between about 10% and about 55% by volume selected from the group consisting of methanol, ethanol, N-propanol, isopropanol, 2-butanol, n-butanol and isobutanol, and a ketone between about 2% and about 35% by volume selected from the group consisting of acetone, 2-butanone, cyclohexanone, 2-pentanone, 3-pentanone, 2-hexanone, 3-hexanone, methyl isobutyl ketone, cyclobutanone, and cyclopentanone, said alcohol and said ketone being the only effective antimicrobially active components in said antimicrobial composition; and

ii. an inactive agent comprising water, wherein said alcohol and said ketone remain miscible in said water; and

b) contacting said viable microorganisms in said flow path of said microfluidic device with said antimicrobial composition; and

c) killing viable microorganisms in said flow path by greater than a 2-log₁₀ order within a time period of fifteen minutes of contacting said viable microorganisms with said antimicrobial composition.

2. The method of reducing a number of viable microorganisms in the flow path of claim 1, wherein said amount of alcohol is between about 30% and about 50% by volume, and wherein said amount of ketone is between about 10% and about 30% by volume.

3. The method of reducing a number of viable microorganisms in the flow path of claim 1, wherein said antimicrobial composition is selected from the group consisting of:

a) methanol about 50%, acetone about 10%, and water 40% v/v;

b) methanol about 50%, 2-butanone (MEK) about 10%, and water 40% v/v;

c) methanol about 50%, cyclohexanone about 10%, and water 40% v/v;

d) ethanol about 50%, acetone about 10%, and water 40% v/v;

e) ethanol about 50%, 2-butanone (MEK) about 10%, and water 40% v/v;

f) ethanol about 50%, cyclohexanone about 10%, and water 40% v/v;

g) isopropanol about 50%, acetone about 10%, and water 40% v/v;

h) isopropanol about 50%, 2-butanone (MEK) about 10%, and water 40% v/v;

i) isopropanol about 50%, cyclohexanone about 10%, and water 40% v/v;

j) N-propanol about 50%, acetone about 10%, and water 40% v/v;

k) N-propanol about 50%, 2-butanone (MEK) about 10%, and water 40% v/v;

l) N-propanol about 50%, cyclohexanone about 10%, and water 40% v/v;

m) 2-butanol (SBA) about 50%, acetone about 10%, and water 40% v/v;

n) 2-butanol (SBA) about 50%, 2-butanone (MEK) about 10%, and water 40% v/v;

o) 2-butanol (SBA) about 50%, cyclohexanone about 10%, and water 40% v/v;

p) methanol about 30%, acetone about 30%, and water 40% v/v;

q) methanol about 30%, 2-butanone (MEK) about 30%, and water 40% v/v;

r) methanol about 30%, cyclohexanone about 30%, and water 40% v/v;

s) ethanol about 30%, acetone about 30%, and water 40% v/v;

t) ethanol about 30%, 2-butanone (MEK) about 30%, and water 40% v/v;

u) ethanol about 30%, cyclohexanone about 30%, and water 40% v/v;

v) isopropanol about 30%, acetone about 30%, and water 40% v/v;

w) isopropanol about 30%, 2-butanone (MEK) about 30%, and water 40% v/v;

x) isopropanol about 30%, cyclohexanone about 30%, and water 40% v/v;

y) N-propanol about 30%, acetone about 30%, and water 40% v/v;

z) N-propanol about 30%, 2-butanone (MEK) about 30%, and water 40% v/v;

aa) N-propanol about 30%, cyclohexanone about 30%, and water 40% v/v;

bb) 2-butanol (SBA) about 30%, acetone about 30%, and water 40% v/v;

cc) 2-butanol (SBA) about 30%, 2-butanone (MEK) about 30%, and water 40% v/v; and

dd) 2-butanol (SBA) about 30%, cyclohexanone about 30%, and water 40% v/v.

4. The method of reducing a number of viable microorganisms in the flow path of claim 1, further comprising the steps of:

a) flowing a first fluid within said flow path prior to flowing a second fluid within said flow path, said second fluid being said antimicrobial composition;

b) displacing said first fluid by flowing a sufficient amount of said antimicrobial composition through said flow path; and

c) flushing the flow path with said antimicrobial composition for a period of time between about 30 seconds and ten minutes.

5. The method of reducing a number of viable microorganisms in the flow path of claim 4, wherein said period of time of contacting said viable microorganisms with said antimicrobial composition is less than five minutes.

6. The method of reducing a number of viable microorganisms in a flow path of a microfluidic device of claim 1, wherein said microfluidic device comprises a flow cytometer.

7. The method of reducing a number of viable microorganisms in a flow path of a microfluidic device of claim 1, wherein said step of flowing an amount of an antimicrobial composition within said flow path comprises the step of flowing an amount of an antimicrobial composition, comprising:

i. 2-butanol between about 5% and about 70% by volume;

ii. 2-butanone between about 1.7% and about 35% by volume; and

iii. water, wherein said alcohol and said ketone remain miscible in said water.

8. The method of reducing a number of viable microorganisms in a flow path of a microfluidic device of claim 7, wherein said antimicrobial composition is selected from the group consisting of:

a) 2-butanol about 5%, 2-butanone about 5%, and water 90% v/v;

b) 2-butanol about 7.5%, 2-butanone about 7.5%, and water 85% v/v;

c) 2-butanol about 8.3%, 2-butanone about 1.7%, and water 90% v/v;

d) 2-butanol about 10%, 2-butanone about 10%, and water 80% v/v;

e) 2-butanol about 12.5%, 2-butanone about 2.5%, and water 85% v/v

f) 2-butanol about 16.5%, 2-butanone about 3.5%, and water 80% v/v;

g) 2-butanol about 30%, 2-butanone about 30%, and water 40% v/v; and

h) 2-butanol about 50%, 2-butanone about 10%, and water 40% v/v.