Spectral imaging of biological samples
First Claim
Patent Images
1. An apparatus comprising:
- a sample holder configured to support a deep tissue sample;
an illumination source to illuminate the sample;
a detector positioned to detect light from the sample; and
an electronic processor coupled to the detector, wherein the electronic processor is configured to;
(i) provide spectrally resolved information about light coming from different spatial locations of a sample, wherein the light includes contributions from different components in the sample;
(ii) decompose at least some of the spectrally resolved information for each of at least some of the different spatial locations into contributions from spectral estimates associated with at least some of the components in the sample; and
(iii) construct a deep tissue image of the sample based on the decomposition to preferentially show a selected one of the components,wherein the processor determines at least a first one of the spectral estimates for a first one of the components from at least part of the spectrally resolved information using an unsupervised classification technique.
1 Assignment
0 Petitions
Accused Products
Abstract
The invention features a method including: (i) providing spectrally resolved information about light coming from different spatial locations in a sample comprising deep tissue in response to an illumination of the sample, wherein the light includes contributions from different components in the sample; (ii) decomposing the spectrally resolved information for each of at least some of the different spatial locations into contributions from spectral estimates associated with at least some of the components in the sample; and (iii) constructing a deep tissue image of the sample based on the decomposition to preferentially show a selected one of the components.
110 Citations
50 Claims
-
1. An apparatus comprising:
-
a sample holder configured to support a deep tissue sample; an illumination source to illuminate the sample; a detector positioned to detect light from the sample; and an electronic processor coupled to the detector, wherein the electronic processor is configured to;
(i) provide spectrally resolved information about light coming from different spatial locations of a sample, wherein the light includes contributions from different components in the sample;
(ii) decompose at least some of the spectrally resolved information for each of at least some of the different spatial locations into contributions from spectral estimates associated with at least some of the components in the sample; and
(iii) construct a deep tissue image of the sample based on the decomposition to preferentially show a selected one of the components,wherein the processor determines at least a first one of the spectral estimates for a first one of the components from at least part of the spectrally resolved information using an unsupervised classification technique. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
-
-
27. An apparatus comprising:
-
a sample holder configured to support a sample; an illumination source to illuminate the sample; a detector positioned to detect light from the sample; and an electronic processor coupled to the detector, wherein the electronic processor is configured to;
(i) provide spectrally resolved information about light coming from different spatial locations of a sample, wherein the light includes contributions from different components in the sample;
(ii) decompose at least some of the spectrally resolved information for each of at least some of the different spatial locations into a contribution from a spectral estimate of a pure spectrum for at least a first one of the components in the sample; and
(iii) construct an image of the sample based on the decomposition to preferentially show a selected one of the components, wherein the processor derives the estimate of the pure spectrum for the first component from at least part of the spectrally resolved information corresponding to a first set of one or more of the different spatial locations and a spectral estimate of a pure spectrum for a second one of the components by using an unsupervised classification technique. - View Dependent Claims (28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46)
-
-
47. An apparatus comprising:
-
a sample holder; an illumination source to illuminate the sample; a detector positioned to detect light from the sample; and an electronic processor coupled to the detector, wherein the electronic processor is configured to;
(i) provide spectrally resolved information about light coming from different spatial locations of a sample, wherein the light includes contributions from different components in the sample;
(ii) decompose at least some of the spectrally resolved information for each of at least some of the different spatial locations into contributions from spectral estimates associated with at least some of the components in the sample; and
(iii) construct an image of the sample based on the decomposition to preferentially show a selected one of the components, wherein the decomposition by the electronic processor comprises a first decomposition of the spectrally resolved information at multiple spatial locations into contributions from initial spectral estimates associated with at least some of the components in the sample, improving an accuracy of at least some of the initial spectral estimates based on the first decomposition, and at least a second decomposition of the spectrally resolved information at multiple spatial locations into contributions from the improved spectral estimates. - View Dependent Claims (48, 49, 50)
-
Specification