Method and material for enhanced tissue-biomaterial integration

• US 8,883,182 B2
• Filed: 10/09/2004
• Issued: 11/11/2014
• Est. Priority Date: 10/09/2002
• Status: Active Grant

• Current Assignee: Synthasome, Inc.
• Original Assignee: Synthasome, Inc.
• Inventors: Ratcliffe, Anthony, Elisseeff, Jennifer H., Wang, Dongan
• Primary Examiner(s): SCHUBERG, LAURA J
• Application Number:
  US10/962,278
• Publication Number:
  US 20050196377A1
• Time in Patent Office: 3,685 Days
• Field of Search: None
• US Class Current: 424/423
• CPC Class Codes: A61K 35/32 Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
• CPC Class Codes: A61K 45/06 Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• CPC Class Codes: A61L 24/0005 Ingredients of undetermined constitution or reaction products thereof
• CPC Class Codes: A61L 24/0031 Hydrogels or hydrocolloids
• CPC Class Codes: A61L 27/3604 characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
• CPC Class Codes: A61L 27/3616 Blood, e.g. platelet-rich plasma
• CPC Class Codes: A61L 27/3633 Extracellular matrix [ECM]
• CPC Class Codes: A61L 27/3683 subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
• CPC Class Codes: A61L 27/3687 characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
• CPC Class Codes: A61L 27/3691 characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation
• CPC Class Codes: A61L 27/38 containing added animal cells organs or tissue containing native cells A61L27/36
• CPC Class Codes: A61L 27/50 Materials characterised by their function or physical properties , e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
• CPC Class Codes: A61L 27/52 Hydrogels or hydrocolloids
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: C12N 2533/30 Synthetic polymers thermoreactive polymers, e.g. PNIPAm, C12N2539/10
• CPC Class Codes: C12N 5/0062 General methods for three-dimensional culture
• CPC Class Codes: C12N 5/0655 Chondrocytes; Cartilage
• CPC Class Codes: C12N 5/0697 Artificial constructs associating cells of different lineages, e.g. tissue equivalents blood vessels C12N5/0691

Abstract

The present invention provides a composition and method for the covalent binding of a hydrogel to an extracellular matrix (ECM). Therapeutic applications include tissue repair and delivery of drugs or cells.

Claims

1. A method of joining a first tissue surface with a second tissue surface, the method comprising:

priming the first and second tissue surfaces by reacting the surfaces with a solution containing hydrogen peroxide under ultraviolet irradiation to create a primed first and second tissue surfaces;

removing the unreacted hydrogen peroxide from the first and second primed tissue surfaces;

adding to the primed first and second tissue surfaces a solution of a polymerizable agent wherein the solution containing the polymerizable agent does not contain a photoinitiator other than the primed first and second tissue surfaces; and

exposing the primed first and second tissue surfaces and the polymerizable agent in the absence of photoinitiators to a source of ultraviolet radiation to create a hydrogel directly covalently bound to and bridging the first and second tissue surfaces.

2. A method as in claim 1 wherein the first and second tissue surfaces are cartilage.

3. A method as in claim 1 wherein the first and second tissue surfaces are ligament.

4. A method as in claim 1 wherein the first and second tissue surfaces are tendon.

5. A method as in claim 1 wherein the first and second tissue surfaces are meniscus.

6. A method as in claim 1 wherein the first and second tissue surfaces are periosteum.

7. A method as in claim 1 wherein the first and second tissue surfaces are bone.

8. A method as in claim 1 wherein the first and second tissue surfaces are at least one tendon of the rotator cuff.

9. The method of claim 1, wherein said polymerizable agent has at least one free radical polymerizable group selected from the group consisting of an acrylate, a diacrylate, oligoacrylate, dimethacrylate, and oligomethacrylate.

10. The method of claim 1, wherein a diffusible drug substance is added with said polymerizable agent.

11. The method of claim 1, wherein a plurality of living cells are added with said polymerizable agent.

12. The method of claim 11, wherein the plurality of living cells added with said polymerizable agent are genetically engineered to secrete a therapeutic protein.

13. The method of claim 11, wherein the plurality of living cells added with said polymerizable agent are genetically engineered to secrete an angiogenic factor.