Systems and methods for isolating and using clinically safe adipose derived regenerative cells
First Claim
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1. An automated cell processing unit for isolating a concentrated cell population comprising CD31 positive cells from adipose tissue, wherein said cell processing unit is configured to maintain a closed system, comprising:
- a tissue collection chamber that is configured to receive a first portion of unprocessed adipose tissue from said subject while maintaining a closed system, wherein said tissue collection chamber comprises an aspiration port;
a first filter that is disposed within said tissue collection chamber, wherein said first filter is configured to substantially retain a first component of said unprocessed adipose tissue and substantially pass a second component of said unprocessed adipose tissue, such that said first filter substantially separates said first component from said second component, and wherein said first component comprises a cell population that comprises adipose-derived stem cells and said second component comprises lipid, blood, mature adipocytes and washing solution;
a processing chamber that is joined to said tissue collection chamber by a conduit such that a closed system is maintained, wherein said processing chamber comprises a first filter, a centrifuge, or a spinning membrane filter, or any combination thereof;
a programmable processing device capable of communication with said tissue collection chamber and said processing chamber, wherein said processing device operates a vacuum or a pump configured to use pressure to control ingress and egress of tissues through said automated cell processing unit; and
an outlet wherein said outlet is configured to allow the aseptic removal of a concentrated population comprising CD31 positive cells obtained from said processing chamber.
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Abstract
Systems and methods are described that are used to separate cells from a wide variety of tissues. In particular, automated systems and methods are described that separate regenerative cells, e.g., stem and/or progenitor cells, from adipose tissue. The systems and methods described herein provide rapid and reliable methods of separating and concentrating regenerative cells suitable for re-infusion into a subject.
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Citations
27 Claims
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1. An automated cell processing unit for isolating a concentrated cell population comprising CD31 positive cells from adipose tissue, wherein said cell processing unit is configured to maintain a closed system, comprising:
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a tissue collection chamber that is configured to receive a first portion of unprocessed adipose tissue from said subject while maintaining a closed system, wherein said tissue collection chamber comprises an aspiration port; a first filter that is disposed within said tissue collection chamber, wherein said first filter is configured to substantially retain a first component of said unprocessed adipose tissue and substantially pass a second component of said unprocessed adipose tissue, such that said first filter substantially separates said first component from said second component, and wherein said first component comprises a cell population that comprises adipose-derived stem cells and said second component comprises lipid, blood, mature adipocytes and washing solution; a processing chamber that is joined to said tissue collection chamber by a conduit such that a closed system is maintained, wherein said processing chamber comprises a first filter, a centrifuge, or a spinning membrane filter, or any combination thereof; a programmable processing device capable of communication with said tissue collection chamber and said processing chamber, wherein said processing device operates a vacuum or a pump configured to use pressure to control ingress and egress of tissues through said automated cell processing unit; and an outlet wherein said outlet is configured to allow the aseptic removal of a concentrated population comprising CD31 positive cells obtained from said processing chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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Specification