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Droplet-based particle sorting

  • US 8,883,513 B2
  • Filed: 02/18/2011
  • Issued: 11/11/2014
  • Est. Priority Date: 04/18/2006
  • Status: Expired due to Fees
First Claim
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1. A method of providing a droplet comprising a single particle, the method comprising:

  • (a) providing a droplet comprising a suspension of particles on an electrowetting droplet actuator surrounded by an oil medium;

    (b) dispensing from the droplet of (a) to provide a dispensed droplet, wherein the dispensing is electrowetting-mediated without the use of dielectrophoresis;

    (c) determining whether the dispensed droplet on the electrowetting droplet actuator comprises a single particle; and

    (d) sorting the dispensed droplet on the electrowetting droplet actuator based on the results of the determining step using electrowetting-mediated droplet operations.

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