Method for production of carotenoid-synthesizing microorganism and method for production of carotenoid

• US 8,883,969 B2
• Filed: 09/25/2006
• Issued: 11/11/2014
• Est. Priority Date: 10/28/2005
• Status: Expired due to Fees

• Current Assignee: Tosoh Corporation
• Original Assignee: Tosoh Corporation
• Inventors: Ide, Teruhiko, Tanaka, Toru
• Primary Examiner(s): Joike, Michele K
• Application Number:
  US12/089,486
• Publication Number:
  US 20090226582A1
• Time in Patent Office: 2,969 Days
• US Class Current: 530/350
• CPC Class Codes: A23K 20/179 Colouring agents, e.g. pigmenting or dyeing agents
• CPC Class Codes: A23L 5/44 using carotenoids or xanthophylls
• CPC Class Codes: C12N 15/743 for Agrobacterium; Rhizobium; Bradyrhizobium
• CPC Class Codes: C12P 23/00 Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes containing heterorings C12P17/00

Abstract

A method for producing a carotenoid comprising the steps of cultivating a cell transformed with a DNA sequence comprising a DNA sequence depicted in anyone of SEQ ID NOs: 2-7 or a cell transformed with a vector having a DNA sequence depicted in anyone of SEQ ID NOs: 2-7 under proper culture conditions and isolating the carotenoid from the cell or the culture.

Claims

1. A method of preparing a carotenoid comprising the steps of:

culturing a cell transformed with a DNA chain having a DNA sequence selected from the group consisting of the following (a) to (f) and having a DNA sequence described in SEQ ID NO;

19, SEQ ID NO;

20, or SEQ ID NO;

21 or a cell transformed with a vector having a DNA sequence selected from the group consisting of the following (a) to (f) in an appropriate culture condition and having a DNA sequence described in SEQ ID NO;

19, SEQ ID NO;

20, or SEQ ID NO;

21, and

isolating the carotenoid from the cell or a culture medium;

(a) a DNA sequence encoding a polypeptide having an enzymatic activity of an enzyme that is found in Paracoccus sp. strain MBIC1143 and converts a methylene group at 4 position in β

-ionone ring into a keto group (crtW), described in SEQ ID NO;

2;

(b) a DNA sequence encoding a polypeptide having an enzymatic activity of an enzyme that is found in Paracoccus sp. strain MBIC1143 and adds one hydroxyl group to a carbon at 3-position of 4-keto-β

-ionone ring and/or at 3-position of β

-ionone ring (crtZ), described in SEQ ID NO;

3;

(c) a DNA sequence encoding a polypeptide having an enzymatic activity of an enzyme that is found in Paracoccus sp. strain MBIC1143 and converts lycopene into β

-carotene (crtY), described in SEQ ID NO;

4;

(d) a DNA sequence encoding a polypeptide having an enzymatic activity of an enzyme that is found in Paracoccus sp. strain MBIC1143 and converts phytoene into lycopene (crtI), described in SEQ ID NO;

5;

(e) a DNA sequence encoding a polypeptide having an enzymatic activity of prephytoene synthase that is found in Paracoccus sp. strain MBIC1143 (crtB), as described in SEQ ID NO;

6; and

(f) a DNA sequence encoding a polypeptide having an enzymatic activity of geranylgeranyl diphosphate synthase that is found in Paracoccus sp. strain MBIC1143 (crtE), as described in SEQ ID NO;

7.

4. A continuous oligonucleotide sequence selected from the group consisting of combinations of the DNA chain according to claim 1 and the DNA chain described in SEQ ID NO:

19, SEQ ID NO;

20, or SEQ ID NO;

21.

5. A plasmid vector comprising the oligonucleotide according to claim 4.

6. A cell transformed with the plasmid vector according to claim 5.

7. A method of producing a carotenoid, comprising:

culturing under a condition that enables expression of a polypeptide encoded by the DNA chain sequence of the plasmid vector according to claim 5; and

isolating the carotenoid from a cell or a culture medium of cell.

8. The method according to claim 7, wherein the cell is a prokaryotic host cell.

9. The method according to claim 7, wherein the cell is a marine bacterium.

10. The method according to claim 7, wherein the cell is a bacterium in which 16S rRNA has a DNA base sequence that has a 97% or higher homology to the DNA base sequence of SEQ ID NO:

24.

11. A method of preparing a desired carotenoid or a carotenoid mixture, comprising:

culturing the cell according to claim 6 under an appropriate culture condition;

isolating the desired carotenoid or a carotenoid mixture from the cell or culture medium; and

separating a single carotenoid from other possibly coexisting carotenoids when such a single carotenoid is desired.

12. A method of preparing a food or feed composition, comprising:

executing the method according to claim 11, and then

adding the carotenoid or carotenoid mixture to food or feed.

13. The method according to claim 9, wherein the marine bacterium is Paracoccus sp.

14. The method according to claim 8, wherein the prokaryotic host cell is Escherichia coli or Bacillus.

2. A DNA chain having a promoter activity in a marine bacterium, described in SEQ ID NO:

20.

3. A DNA chain having a promoter activity in a marine bacterium, described in SEQ ID NO:

21.