Multimode cells and methods of using them

• US 8,884,217 B2
• Filed: 04/01/2013
• Issued: 11/11/2014
• Est. Priority Date: 02/26/2010
• Status: Active Grant

• Current Assignee: Perkin Elmer Life Sciences
• Original Assignee: Perkinelmer Health Sciences Incorporated (Perkinelmer Incorporated)
• Inventors: Badiei, Hamid, Kahen, Kaveh
• Primary Examiner(s): BERMAN, JACK I
• Application Number:
  US13/854,458
• Publication Number:
  US 20130284917A1
• Time in Patent Office: 589 Days
• Field of Search: 250281-300
• US Class Current: 250/281
• CPC Class Codes: G01N 30/16 Injection G01N30/24 takes precedence
• CPC Class Codes: G01N 30/24 Automatic injection systems
• CPC Class Codes: G01N 30/6026 Fluid seals
• CPC Class Codes: H01J 49/005 by collision with gas, e.g. by introducing gas or by accelerating ions with an electric field
• CPC Class Codes: H01J 49/24 Vacuum systems, e.g. maintaining desired pressures

Abstract

A mass spectrometer system is provided that is configurable for operation in both a Kinetic Energy Discrimination (KED) and Dynamic Reaction Cell (DRC). A pressurized or collision cell included in the mass spectrometer encloses a quadrupole and is coupled to a source of both inert and reactive gas. To operate in the KED mode, the collision cell can be filled with a quantity of the inert gas and an energy barrier formed between the collision cell and a downstream mass analyzer. Interferer ions collided with the inert gas can lose on average more energy relative to analyte ions of the same mass to charge ratio and can thus be trapped by the energy barrier in greater proportions. To operate instead in the DRC mode, the collision cell can be filled with a quantity of gas that is reactive with the interferer ions only. Mass filtering of the product ions can then transmit proportionally more of the analyte ions to the downstream mass analyzer. A mode controller coordinates the two modes of operation.

Claims

1. A system comprising:

an ion source;

a cell coupled to the ion source and configured to operate in at least three different modes comprising a collision mode, a reaction mode and a standard mode, the three different modes each configured to select analyte ions from a plurality of ions introduced into the cell from the ion source, the cell configured to couple to the ion source at an entrance aperture to permit introduction of the plurality of ions from the ion source into the cell, the cell further comprising a gas inlet configured to receive a substantially inert gas to pressurize the cell in a collision mode and to receive a reactive gas to pressurize the cell in a reaction mode, the pressurized cell further comprising an exit aperture configured to provide the analyte ions from the cell;

a mass analyzer coupled to the cell; and

a controller electrically coupled to the cell and configured to provide the substantially inert gas to pressurize the cell in the collision mode, configured to provide the reactive gas to pressurize the cell in the reaction mode, and configured to maintain the cell under vacuum in the standard mode, in which the controller is configured to provide an effective voltage from a voltage source to the cell in the collision mode to select ions comprising an energy greater than a barrier energy and an effective voltage from the voltage source in the reaction mode to select ions using mass filtering.

2. The system of claim 1, in which the pressurized cell comprises a quadrupole.

3. The system of claim 2, in which the voltage is provided to the quadrupole to provide a quadrupolar field effective to confine a substantial amount of non-analyte ions in the plurality of introduced ions by colliding the non-analyte ions with the substantially inert gas in the collision mode.

4. The system of claim 3, further comprising axial electrodes configured to provide an axial field to direct the analyte ions from the entrance aperture toward an exit aperture of the pressurized cell.

5. The system of claim 4, in which the axial field strength has an axial field gradient between 0.1 V/cm and 0.5 V/cm.

6. The system of claim 2, further comprising an exit member proximal to an exit aperture of the pressurized cell, the exit member comprising an exit potential to attract analyte ions toward the exit aperture of the pressurized cell.

7. The system of claim 6, in which the exit potential is between about −

60 Volts and −

18 Volts in the collision mode.

8. The system of claim 6, in which the exit potential is between about −

20 Volts and 0 Volts in the reaction mode.

9. The system of claim 6, further comprising an entrance member proximal to the entrance aperture of the pressurized cell, the entrance member comprising an entrance potential more positive than the exit potential in the collision mode.

10. The system of claim 9, in which the entrance potential is between −

10 Volts and +2 Volts.

11. The system of claim 6, further comprising an entrance member proximal to the entrance aperture of the pressurized cell, the entrance member comprising an entrance potential substantially the same as the exit potential in the reaction mode.

12. The system of claim 11, in which the exit member comprises a potential between −

60 Volts and −

18 Volts in the collision mode.

13. The system of claim 11, in which the exit member comprises a potential between −

20 Volts and 0 Volts in the reaction mode.

14. The system of claim 1, in which the mass analyzer is positioned between the ion source and the cell.

15. The system of claim 1, in which the mass analyzer is positioned downstream from the cell.

16. The system of claim 1, further comprising a detector coupled to the cell.

17. The system of claim 1, in which the ion source is configured as an inductively coupled plasma.

18. The system of claim 1, further comprising an additional cell coupled to the cell, the additional cell configured to operate in at least three different modes comprising a collision mode, a reaction mode and a standard mode.

19. The system of claim 18, in which the additional cell is configured to operate in a standard mode when the cell is being operated in the collision gas mode or the reaction mode.