Microfluidic channel for removing bubbles in fluid

• US 8,889,085 B2
• Filed: 11/23/2011
• Issued: 11/18/2014
• Est. Priority Date: 06/29/2011
• Status: Expired due to Fees

• Current Assignee: Samsung Electronics Co. Ltd.
• Original Assignee: Samsung Electronics Co. Ltd.
• Inventors: Lee, Woochang, Jung, Jae Yeon, Kim, Sang Kyu, Lee, Soo Suk, Choi, Youn Suk, Hahn, Young Ki
• Primary Examiner(s): Hyun, Paul
• Application Number:
  US13/303,503
• Publication Number:
  US 20130004385A1
• Time in Patent Office: 1,091 Days
• Field of Search: 422502-506, 436/180
• US Class Current: 422/506
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2200/0684 Venting, avoiding backpressure, avoid gas bubbles
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 2400/043 magnetic forces
• CPC Class Codes: B01L 2400/0677 phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
• CPC Class Codes: B01L 3/502723 characterised by venting arrangements
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A microfluidic channel for effectively removing a gas from a fluid, and microfluidic apparatus including the same are provided. The microfluidic channel includes a first channel having a uniform cross-sectional area, and a second channel connected to the first channel and having a gradually expanded cross-sectional area.

Claims

1. A microfluidic apparatus with a channel that removes bubbles from a fluid, the apparatus comprising:

a substrate with an axis of rotation;

a first channel having an upper wall, a lower wall opposite the upper wall, and a uniform cross-sectional area, wherein the upper wall is nearer the axis of rotation than the lower wall;

a second channel in fluid connection with the first channel, wherein the second channel has an upper wall extending from the upper wall of the first channel, a lower wall opposite the upper wall, and a cross-sectional area which increases in a flow direction away from the first channel; and

a ventilation unit in fluid connection with the second channel, which extends outside the second channel from the terminal end of the upper wall of the second channel towards the axis of rotation.

2. The microfluidic apparatus of claim 1, wherein the material of the first and second channels comprises glass, silicon, silicon rubber, isobonyl acrylate, polyethylene terephthalate, polydimethylsiloxane, poly methyl methacrylate, polycarbonate, polypropylene, polystyrene, polyvinyl chloride, polysiloxane, polyimide and polyurethane, or any combination thereof.

3. The microfluidic apparatus of claim 1, wherein the apparatus is manufactured by a lamination process, a bonding process using an adhesive and surface reformation, or an ultrasonic fusion process.

4. The microfluidic apparatus of claim 1, further comprising a barrier positioned to hinder fluid flow from the first channel to the second channel, wherein the barrier is disposed at an end of the first channel adjacent the second channel, or within the second channel.

5. The microfluidic apparatus of claim 4, wherein the barrier includes polycaprolactone, polystyrene, propylene carbonate, ethylene carbonate, dimethylcarbonate, diethylcarbonate, dibutyl phthalate, dioctyl phthalate, diisooctyl phthalate, diheptylnonyl phthalate, tritolylphospate and dioctyl adipate, or any combination thereof.

6. The microfluidic apparatus of claim 1, wherein the ventilation unit has a curved shape.

7. The microfluidic apparatus of claim 1 further comprising:

a fluid injector;

a fluid container which is in fluid connection with the fluid injector, wherein the first channel of the microfluidic apparatus is in fluid connection with the fluid container; and

a valve which controls fluid flow from the fluid container to the first channel.

8. The microfluidic apparatus of claim 7, further comprising a barrier positioned to hinder fluid flow from the first channel to the second channel, wherein the barrier is disposed at an end of the first channel adjacent the second channel, or within the second channel.

9. The microfluidic apparatus of claim 8, wherein the barrier includes polycaprolactone, polystyrene, propylene carbonate, ethylene carbonate, dimethylcarbonate, diethylcarbonate, dibutyl phthalate, dioctyl phthalate, diisooctyl phthalate, diheptylnonyl phthalate, tritolylphospate and dioctyl adipate, or any combination thereof.

10. The microfluidic apparatus of claim 7, wherein the ventilation unit extends from the terminal end of the upper wall of the second channel towards the axis of rotation.

11. The microfluidic apparatus of claim 10, wherein the ventilation unit has a curved shape.

12. A method of removing bubbles from a fluid comprising introducing a fluid into the first channel of the microfluidic apparatus of claim 1 and rotating the apparatus about the axis of rotation to flow fluid through the first and second channels.

13. The method of claim 12, wherein the fluid comprises protein, deoxyribonucleic acid, ribonucleic acid, peptides, carbohydrates, bacteria, plants, mold, animal cells, or any combination thereof.

14. A method of removing bubbles from a fluid comprising introducing a fluid into the first channel of the microfluidic apparatus of claim 7 and rotating the apparatus about the axis of rotation to flow fluid through the first and second channels.

15. The method of claim 14, wherein the fluid comprises protein, deoxyribonucleic acid, ribonucleic acid, peptides, carbohydrates, bacteria, plants, mold, animal cells, or any combination thereof.

16. The method of claim 12, wherein the fluid comprises a gas bubble and the flow of the fluid through the first channel toward the second channel removes the gas bubble from the fluid.

17. The method of claim 14, wherein the fluid comprises a gas bubble and the flow of the fluid through the first channel toward the second channel removes the gas bubble from the fluid.