Stem cell capture and immobilization coatings for medical devices and implants

• US 8,889,168 B2
• Filed: 08/07/2009
• Issued: 11/18/2014
• Est. Priority Date: 08/07/2008
• Status: Active Grant

• Current Assignee: Bioactive Surgical, Inc.
• Original Assignee: Bioactive Surgical, Inc.
• Inventors: Spedden, Richard H., Qiu, Judy, Borch, William
• Primary Examiner(s): HILL, KEVIN KAI
• Application Number:
  US12/537,409
• Publication Number:
  US 20100034864A1
• Time in Patent Office: 1,929 Days
• Field of Search: 424/93.1, 424/93.7, 424/423
• US Class Current: 424/423
• CPC Class Codes: A61L 27/227 Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
• CPC Class Codes: A61L 27/38 containing added animal cells organs or tissue containing native cells A61L27/36
• CPC Class Codes: A61L 29/005 Ingredients of undetermined constitution or reaction products thereof
• CPC Class Codes: A61L 29/048 Other specific proteins or polypeptides not covered by A61L29/045 - A61L29/047
• CPC Class Codes: A61L 31/005 Ingredients of undetermined constitution or reaction products thereof
• CPC Class Codes: A61L 31/047 Other specific proteins or polypeptides not covered by A61L31/044 - A61L31/046
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 19/08 for bone diseases, e.g. rachitism, Paget's disease

Abstract

Constructs and methods for immobilizing stem and other precursor cells, as well as other bioactive materials of therapeutic value on the surfaces of medical devices, such as bone, cartilage, spinal and tooth implants, are described herein. The present invention has broad application in the incorporation of bioactive and therapeutic materials in or on a medical implant or other interventional device, having particular value in enabling the real-time, utilization by medical personnel of bioactive materials extracted from the patient and subsequently reintroduced and immobilized in an implant device.

Claims

1. A porous biocompatible medical implant device comprising a solid outer surface provided with a plurality of pores in which one or more micelle-target cell units are disposed, wherein said micelle-target cell units comprise (a) at least one target cell bound to (b) a non-polar-liquid filled micelle or micelle-like construct assembled from (i) a non-polar liquid, (ii) a polar liquid and (ii) a plurality of amphiphilic molecules comprised of a hydrophobic tail and a hydrophilic head, wherein said hydrophilic head binds a surface moiety of said target cell.

2. The device of claim 1, wherein the target cell is selected from the group consisting of stem cells, precursor or progenitor cells, osteoblasts and tissue cells.

3. The device of claim 1, wherein said hydrophilic head comprises a nucleic acid aptamer conjugated to a hydrocarbon chain of the form (CH2)n where n is greater than eight.

4. The device of claim 1, wherein at least one of said amphiphilic molecules comprises a conjugation of biotin, avidin and either an aptamer or antibody.

5. The device of claim 1, wherein said plurality of amphiphilic molecules comprises two or more different targeting moieties, each of which has specificity for different features on the same cell.

7. The device of claim 1, wherein said hydrophilic head comprises a nucleic acid aptamer.

6. A target-cell carrying biocompatible medical implant device fabricated by a method comprising the steps of:

a. providing a porous biocompatible medical implant comprising a solid exterior surface having a plurality of pores;

b. providing a solution comprised of the following components;

(i) a non-polar liquid, (ii) a polar liquid and (ii) a plurality of amphiphilic molecules composed of hydrophobic tail ends and hydrophilic head ends, wherein said head ends have a binding affinity for one or more target cells, further wherein said solution components assemble into a non-polar-liquid-filled micelles or micelle-like constructs;

c. contacting said micelles or micelle-like constructs with a solution containing target cells to which the hydrophilic head ends of said amphiphilic molecules having a binding affinity and inducing said micelles or micelle-like constructs to bind said target cells and form micelle-target cell units; and

d. introducing said micelle-target cell units into the pores of said implant exterior surface so as to yield a biocompatible medical implant device having target cells disposed within said exterior surface pores.