Enzyme catalyst immobilized on porous fluoropolymer support

• US 8,889,373 B2
• Filed: 08/12/2010
• Issued: 11/18/2014
• Est. Priority Date: 08/12/2010
• Status: Active Grant

• Current Assignee: Eastman Chemical Company
• Original Assignee: Eastman Chemical Company
• Inventors: Clendennen, Stephanie Kay
• Primary Examiner(s): NOAKES, SUZANNE MARIE
• Application Number:
  US12/855,117
• Publication Number:
  US 20120040395A1
• Time in Patent Office: 1,559 Days
• Field of Search: None
• US Class Current: 435/44
• CPC Class Codes: C12N 11/04 entrapped within the carrier, e.g. gel or hollow fibres
• CPC Class Codes: C12N 11/082 obtained by reactions only involving carbon-to-carbon unsaturated bonds

Abstract

Enzyme catalyst immobilized on porous fluoropolymer supports. Catalytically active enzymes can be bound to a variety of fluoropolymer supports for use as a reaction catalyst. Moreover, consistently high rate of conversion during catalyst re-use over time is achieved. Furthermore, inactive enzyme catalyst can be stripped from the support and fresh enzyme can be bound to the support to achieve the original conversion rate. The immobilization of catalytic enzymes on porous fluoropolymers is a viable and novel technology for the preparation of advantaged catalysts.

Claims

1. An immobilized catalyst comprising a porous fluoropolymer support having at least one layer and at least one enzyme catalyst immobilized via non-covalently binding the enzyme catalyst to the support,

wherein the support is selected from the group consisting of a membrane, a film, a tape, a fiber, a hollow fiber, a tube, a braid, and a sponge,

the effective pore size of each layer of the support is greater than the size of the enzyme catalyst, and

the immobilized catalyst maintains catalytic activity for between 2 and 50 reuses, and

wherein the enzyme catalyst is selected from the group consisting of hydrolytic enzyme, transferase, isomerase, ligase, dehydratase, catalase, hydroxylase, nitrilase, nuclease, polymerase, kinase, phosphatase and mixtures thereof.

2. The catalyst according to claim 1, wherein the enzyme catalyst is in the form of an isolated enzyme, a mixture of enzymes, a cell extract, or a whole cell.

3. A reactor comprising the immobilized catalyst according to claim 1.

4. The reactor according to claim 3, wherein the reactor comprises a cartridge wherein the cartridge comprises the immobilized catalyst.

5. An immobilized catalyst comprising a porous fluoropolymer membrane and at least one catalyst immobilized via non-covalently binding the enzyme catalyst to the membrane,

wherein the enzyme catalyst is immobilized on both surfaces of the membrane;

and

the effective pore size of each layer of the membrane is greater than the size of the enzyme catalyst, and

the immobilized catalyst maintains catalytic activity for between 2 and 50 reuses, and

wherein the enzyme catalyst is selected from the group consisting of hydrolytic enzyme, transferase, isomerase, ligase, dehydratase, catalase, hydroxylase, nitrilase, nuclease, polymerase, kinase, phosphatase and mixtures thereof.

6. The catalyst according to claim 5, wherein the membrane is symmetrical.

7. The catalyst according to claim 5, wherein the membrane is hydrophobic.

8. The catalyst according to claim 5, wherein the membrane has a single layer.

9. The catalyst according to claim 5, wherein the enzyme catalyst is in the form of an isolated enzyme, a mixture of enzymes, a cell extract, or a whole cell.

10. A cartridge comprising the immobilized catalyst according to claim 5.

11. A reactor comprising the cartridge according to claim 10.

12. A method of producing an immobilized catalyst comprising contacting at least one enzyme catalyst and the support,

wherein the support has at least one layer and is selected from the group consisting of a membrane, a film, a tape, a fiber, a hollow fiber, a tube, a braid, and a sponge;

wherein the enzyme catalyst is immobilized via non-covalently binding the enzyme catalyst to the support; and

the effective pore size of each layer of the support is greater than the size of the enzyme catalyst, and

the immobilized catalyst maintains catalytic activity for between 2 and 50 reuses, and

wherein the enzyme catalyst is selected from the group consisting of hydrolytic enzyme, transferase, isomerase, ligase, dehydratase, catalase, hydroxylase, nitrilase, nuclease, polymerase, kinase, phosphatase and mixtures thereof.

13. The method according to claim 12 further comprising:

stripping the enzyme catalyst from the fluoropolymer support to remove any enzyme catalyst bound to the support, and

contacting the fluoropolymer support with at least one enzyme catalyst.

14. The method according to claim 12, further comprising pre-wetting the support with a pre-wetting agent selected from the group consisting of water, an alcohol, a polyol, and a combination thereof.

15. The method according to claim 14, wherein the pre-wetting agent is removed from the support prior to contacting the support with the enzyme catalyst.

16. The method according to claim 12, wherein the enzyme catalyst is in the form of an isolated enzyme, a mixture of enzymes, a cell extract, or a whole cell.

17. The method according to claim 12, wherein the contacting is from about 0.5 hours to about 144 hours.

18. The method according to claim 17, wherein the contacting is from about 2 hours to about 48 hours.

19. The method according to claim 12, wherein the contacting is at a temperature of from about 0°

C. to about 100°

C.

20. The method according to claim 19, wherein the contacting is at a temperature of from about 4°

C. to about 80°

C.

21. The method according to claim 20, wherein the contacting is at a temperature of from about 15°

C. to about 50°

C.

22. A method of producing a chemical comprising contacting at least one reactant with at least one immobilized catalyst to produce the chemical,

wherein the enzyme catalyst is immobilized via non-covalently binding the enzyme catalyst on a porous fluoropolymer support;

the support has at least one layer and is selected from the group consisting of membrane, a film, a tape, a fiber, a hollow fiber, a tube, a braid, and a sponge; and

the effective pore size of each layer of the support is greater than the size of the enzyme catalyst, and

the immobilized catalyst maintains catalytic activity for between 2 and 50 reuses, and

wherein the enzyme catalyst is selected from the group consisting of hydrolytic enzyme, transferase, isomerase, ligase, dehydratase, catalase, hydroxylase, nitrilase, nuclease, polymerase, kinase, phosphatase and mixtures thereof.

23. The method according to claim 22, wherein the catalyst is in the form of an isolated enzyme, a mixture of enzymes, a cell extract, or a whole cell.