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Microfluidic device

  • US 8,891,084 B2
  • Filed: 07/06/2010
  • Issued: 11/18/2014
  • Est. Priority Date: 07/07/2009
  • Status: Active Grant
First Claim
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1. A method of detecting particles in a sample, the method comprising the steps of:

  • a) providing a microfluidic device, said microfluidic device comprising;

    a substrate;

    a first microfluidic flow channel formed in said substrate, wherein said first flow channel extends through a portion of said substrate adapted to facilitate cytometry analysis of first cells flowing in said first flow channel; and

    a second microfluidic flow channel formed in said substrate, wherein said second flow channel extends through a portion of said substrate adapted to facilitate cytometry analysis of second cells flowing in said second flow channel;

    b) producing an excitation beam aimed at said first and second flow channels;

    c) spatially varying said excitation beam in a first patterned manner prior to said excitation beam reaching said first flow channel such that a first patterned excitation beam illuminates said first flow channel; and

    d) spatially varying said excitation beam in a second patterned manner prior to said excitation beam reaching said second flow channel such that a second patterned excitation beam illuminates said second flow channel.

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