Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
First Claim
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1. A roller bottle comprising primate pluripotent stem cell (pPSC) aggregates in suspension in a physiologically acceptable medium, wherein the pPSC aggregates are formed in the roller bottle.
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Abstract
The present invention relates to methods for production of undifferentiated or differentiated embryonic stem cell aggregate suspension cultures from undifferentiated or differentiated embryonic stem cell single cell suspensions and methods of differentiation thereof.
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22 Claims
- 1. A roller bottle comprising primate pluripotent stem cell (pPSC) aggregates in suspension in a physiologically acceptable medium, wherein the pPSC aggregates are formed in the roller bottle.
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