Treatment of transcription factor E3 (TFE3) and insulin receptor substrate 2(IRS2) related diseases by inhibition of natural antisense transcript to TFE3
First Claim
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1. A method of upregulating a function of and/or the expression of an Transcription factor E3 (TFE3) and/or Insulin Receptor Substrate 2 (IRS2) polynucleotide in cells or tissues in vivo or in vitro comprising:
- contacting, said cells or tissues with at least one antisense oligonucleotide of 15-30 nucleotides in length that specifically targets a region of a natural antisense polynucleotide of the Transcription factor E3 (TFE3) polynucleotide comprising SEQ ID NO;
3;
thereby upregulating a function of and/or the expression of the Transcription factor E3 (TFE3) and/or Insulin Receptor Substrate 2 (IRS2) polynucleotide in cells or tissues in vivo or in vitro.
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Abstract
The present invention relates to antisense oligonucleotides that modulate the expression of and/or function of Transcription factor E3 (TFE3) and/or Insulin Receptor Substrate 2 (IRS2) polynucleotides, in particular, by targeting natural antisense polynucleotides of Transcription factor E3 (TFE3) and/or Insulin Receptor Substrate 2 (IRS2). The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of TFE3 and/or IRS2.
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Citations
12 Claims
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1. A method of upregulating a function of and/or the expression of an Transcription factor E3 (TFE3) and/or Insulin Receptor Substrate 2 (IRS2) polynucleotide in cells or tissues in vivo or in vitro comprising:
contacting, said cells or tissues with at least one antisense oligonucleotide of 15-30 nucleotides in length that specifically targets a region of a natural antisense polynucleotide of the Transcription factor E3 (TFE3) polynucleotide comprising SEQ ID NO;
3;
thereby upregulating a function of and/or the expression of the Transcription factor E3 (TFE3) and/or Insulin Receptor Substrate 2 (IRS2) polynucleotide in cells or tissues in vivo or in vitro.- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method of upregulating a function of and/or the expression of an Transcription factor E3 (TFE3) and/or Insulin Receptor Substrate 2 (IRS2) gene in mammalian cells or tissues in vivo or in vitro comprising:
contacting said cells or tissues with at least one short interfering RNA (siRNA) oligonucleotide 19 to 30 nucleotides in length, said at least one siRNA oligonucleotide being specific for a natural antisense polynucleotide of an Transcription factor E3 (TFE3) polynucleotide selected from SEQ ID NO;
3, wherein said at least one siRNA oligonucleotide has at least 80% sequence complementarity to about 19 consecutive nucleic acids of said natural antisense polynucleotide of the Transcription factor E3 (TFE3) polynucleotide; and
, upregulating a function of and/or the expression of Transcription factor E3 (TFE3) and/or Insulin Receptor Substrate 2 (IRS2) in mammalian cells or tissues in vivo or in vitro.- View Dependent Claims (11)
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12. A method of upregulating a function of and/or the expression of Transcription factor E3 (TFE3) and/or Insulin Receptor Substrate 2 (IRS2) in mammalian cells or tissues in vitro comprising:
contacting said cells or tissues with at least one antisense oligonucleotide of about 15 to 30 nucleotides in length specific for a natural antisense strand of an Transetiption factor E3 (TFE3) polynucleotide selected from SEQ ID NO;
3 wherein said at least one antisense oligonucleotide has at least 80% sequence complementarity to said natural antisense strand thereby upregulating the function and/or expression of the Transcription factor E3 (TFE3) and/or Insulin Receptor Substrate 2 (IRS2) in mammalian cells or tissues in vitro.
Specification