Robust, rapid, secure sample manipulation before during and after ionization for a spectroscopy system

• US 8,901,488 B1
• Filed: 04/18/2012
• Issued: 12/02/2014
• Est. Priority Date: 04/18/2011
• Status: Active Grant

• Current Assignee: Bruker Scientific LLC (Bruker Corporation)
• Original Assignee: IonSense, Inc. (Bruker Corporation)
• Inventors: Musselman, Brian D.
• Primary Examiner(s): MASKELL, MICHAEL P
• Application Number:
  US13/449,615
• Time in Patent Office: 958 Days
• Field of Search: 250/288
• US Class Current: 250/288
• CPC Class Codes: G01N 2560/00 Chemical aspects of mass spectrometric analysis of biological material
• CPC Class Codes: G01N 33/54326 Magnetic particles
• CPC Class Codes: H01J 27/022 Details
• CPC Class Codes: H01J 27/24 using photo-ionisation, e.g. using laser beam
• CPC Class Codes: H01J 27/26 using surface ionisation, e.g. field effect ion sources, thermionic ion sources H01J27/20, H01J27/24 take precedence
• CPC Class Codes: H01J 49/10 Ion sources; Ion guns
• CPC Class Codes: H01J 49/14 using particle bombardment, e.g. ionisation chambers

Abstract

This invention provides for the efficient positioning of a sample to be analyzed by using either magnetic or electro-mechanical fields to retain the sample in the ionization region. In an embodiment of the present invention, the sample is contacted with a sampler device, which is inserted into a chamber and accurately positioned using electro-mechanical devices. In an embodiment of the invention, the influence of an electro-mechanical field on the sampler device enables the sample to be positioned in the ionization region to be contacted by particles that result in ionization of the sample whereby rendering the resulting ions available for analysis.

Claims

1. A device for ionizing an analyte comprising:

a chamber with at least one port to allow the analyte to enter the chamber and adapted to mix the analyte with one or both dry powder and dry granule material;

a magnetic field source adapted to constrain the analyte mixed with the one or both dry powder and dry granule material within the chamber; and

an ionization source directed at the analyte within the chamber mixed with the one or both dry powder and dry granule material to form analyte ions.

2. The device of claim 1, where the position of the analyte can be adjusted relative to the ionization source prior to the ionization event.

3. The device of claim 1, where the position of the analyte can be adjusted relative to the ionization source during the ionization event.

4. The device of claim 1, where the position of the analyte can be adjusted relative to the ionization source after the ionization event.

5. The device of claim 1, where the ionization source is an atmospheric pressure ionization source.

6. The device of claim 5, where the atmospheric pressure ionization is selected from the group consisting of a Direct Ionization in Real Time source, a desorption electrospray ionization (DESI) source, an atmospheric laser desorption ionization source, a Corona discharge source, an inductively coupled plasma (ICP) source and a glow discharge source.

7. The device of claim 1, further comprising a spectroscopic analyzer adapted to analyze the analyte ions.

8. The device of claim 7, where the spectroscopic analyzer is selected from the group consisting of a mass spectrometer, Raman spectrometer, electro-magnetic absorption spectrometer, electro-magnetic emission spectrometer, surface detection spectrometer, differential scanning mobility spectrometer and ion mobility mass spectrometer.

9. The device of claim 1, where the one or both dry powder and dry granule material includes a metal with one or both a magnetic dipole moment and an inducible magnetic dipole moment.

10. The device of claim 1, where the magnetic field source is generated by one or both a magnet and an electro-magnet.

11. A kit for handling a sample for ionization comprising:

(a) a vial adapted to be opened and resealed containing one or both dry powder and dry granule material, where opening the vial and locating the sample in the vial and resealing the vial mixes the sample and the one or both dry powder and dry granule material;

(b) a probe adapted to enter the vial and thereby position the one or both dry powder and dry granule material mixed with the sample onto the probe for removal from the vial; and

(c) an analysis plate with one or both a fixed magnet and an electro-magnet adapted to move the one or both dry powder and dry granule material mixed with the sample from the probe onto the analysis plate while constraining the one or both dry powder and dry granule material mixed with the sample to one or more regions on the plate for ionization.

12. The kit of claim 11, where the probe comprises a proximal end, a distal end, a coil situated at the distal end and a switch, where the switch is adapted to apply or discontinue an electro-magnetic field through the coil to position the one or both dry powder and dry granule material and the sample relative to the probe.

13. The kit of claim 12, where the one or both dry powder and dry granule material is a ferromagnetic metal.

14. A method of ionizing a sample comprising:

(a) mixing the sample with a material consisting essentially of one or both of a dry powder and dry granule material;

(b) controlling the position of the sample mixed with the material in a chamber using one or both an electro-mechanical force and an electric field; and

(c) generating one or more analyte ions of the sample in the chamber while the sample remains inside the chamber.

15. The method of claim 14, where the analyte is selected from the group consisting of a powder, lyophilized sample, plant material, fine grains, and a loose substance.

16. The method of claim 14, further comprising adjusting the position of the analyte relative to the ionization source one or more of prior to, during and after the ionization event.

17. The method of claim 14, where the material and the sample are physically mixed.

18. The method of claim 14, where the one or more ions are generated at atmospheric pressure.

19. The method of claim 14, further comprising directing the ions into a spectroscopic analyzer to analyze the analyte ions.

20. The method of claim 14, where the material is selected from one or more of Fe, FeO, FeOFe₂O₃, Fe₂O₃, MnOFe₂O₃, MgOFe₂O₃, Y₃Fe₅O₁₂, Fe₃O₄, Cu, CuO, CuOFe₂O₃, Cu₂O, Al, Al₂O₃, Ni, NiO, Ni₂O₃, Ni(OH)₂, NiOFe₂O₃, Co, NaCoO₂ and Co₃O₄.