Cationic polymer based wired enzyme formulations for use in analyte sensors

• US 8,906,211 B2
• Filed: 01/27/2014
• Issued: 12/09/2014
• Est. Priority Date: 09/15/2008
• Status: Active Grant

• Current Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories)
• Original Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories)
• Inventors: Feldman, Benjamin J., Ouyang, Tianmei, Liu, Zenghe
• Primary Examiner(s): LEONG, SUSAN DANG
• Application Number:
  US14/165,268
• Publication Number:
  US 20140141487A1
• Time in Patent Office: 316 Days
• Field of Search: 600/345, 600/347, 600/346, 600/348, 600/373, 600/395, 205775-7945, 20440301-40315
• US Class Current: 204/403.12
• CPC Class Codes: A61K 38/28 Insulins
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: C12Q 1/32 involving dehydrogenase
• CPC Class Codes: G01N 2333/904 acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 27/3273 Devices therefor, e.g. test element readers, circuitry details not specific to biochemical electrodes G01N33/4875

Abstract

Embodiments of the invention include analyte-responsive compositions and electrochemical analyte sensors having a sensing layer that includes an analyte-responsive enzyme and a cationic polymer. Also provided are systems and methods of making the sensors and using the electrochemical analyte sensors in analyte monitoring.

Claims

1. A glucose-responsive composition comprising:

a glucose-responsive enzyme, a cationic polymer selected from the group consisting of polyallylamine (PAH), polyethyleneimine (PEI), poly(L-lysine) (PLL), or poly(L-arginine) (PLA), and a positively charged redox mediator non-covalently associated with the cationic polymer.

2. The composition of claim 1, wherein the glucose-responsive enzyme is glucose oxidase (GOx).

3. The composition of claim 1, wherein the glucose-responsive enzyme is glucose dehydrogenase (GDH).

4. The composition of claim 3, wherein the glucose dehydrogenase is associated with a co-factor.

5. The composition of claim 4, wherein the co-factor is flavin adenine dinucleotide (FAD), nicotinamide adenine dinucleotide (NAD), or pyrroloquinoline quinone (PQQ).

6. The composition of claim 1, wherein the glucose-responsive enzyme comprises a complex of glucose dehydrogenase (GDH) and flavin adenine dinucleotide (FAD).

7. The composition of claim 1, wherein the redox mediator comprises ferricyanide, phenanthroline quinone, or ferrocene.

8. The composition of claim 1, wherein the redox mediator comprises a transition metal complex.

9. The composition of claim 8, wherein the transition metal complex comprises osmium.

10. The composition of claim 9, wherein the transition metal complex comprises the following formula:

11. The composition of claim 10, wherein the transition metal complex comprises the following formula:

12. The composition of claim 11, wherein at least one of R₁, R₂, and R′

₁ comprises a reactive group selected from the group consisting of carboxy, activated ester, sulfonyl halide, sulfonate ester, isocyanate, isothiocyanate, epoxide, aziridine, halide, aldehyde, ketone, amine, acrylamide, thiol, acyl azide, acyl halide, hydrazine, hydroxyamine, alkyl halide, imidazole, pyridine, phenol, alkyl sulfonate, halotriazine, imido ester, maleimide, hydrazide, hydroxy, and photo-reactive azido aryl groups.