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Methods for sequencing individual nucleic acids under tension

  • US 8,906,617 B2
  • Filed: 03/09/2011
  • Issued: 12/09/2014
  • Est. Priority Date: 08/08/2008
  • Status: Active Grant
First Claim
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1. A method for sequencing comprising:

  • immobilizing a single stranded nucleic acid target by its 3′

    end or 5′

    end to a surface of a substrate, the single-stranded nucleic acid target including a plurality of tandem repeat nucleic acid sequences, the single-stranded nucleic acid target including a detectable moiety at its free end;

    applying at least one oligonucleotide complementary to at least a portion of a tandem repeat single stranded nucleic acid sequence of the nucleic acid target, the at least one oligonucleotide having a known sequence and hybridizing to the at least a portion of the tandem repeat nucleic acid sequence of the single stranded nucleic acid target;

    applying a force to the single stranded nucleic acid target;

    determining with an ion sensitive field effect transistor array and when the force is applied, a change in a length of the single stranded nucleic acid target arising from hybridizing the at least one oligonucleotide, the determining based on a change in a position of the detectable moiety caused by the conversion of single stranded bases to double stranded bases; and

    associating the change in the length of the single stranded nucleic acid target with a nucleotide sequence for the nucleic acid target.

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