Apparatus and methods for parallel processing of micro-volume liquid reactions
First Claim
1. A method for simultaneously conducting a plurality of polynucleotide reactions, the method comprising:
- (a) providing a microhole apparatus comprising a substrate having a first surface and a second surface and a plurality of holes extending through the substrate from the first surface to the second surface;
(b) introducing a plurality of liquid samples comprising a nucleic acid into the plurality of holes;
(c) coating the first surface, the second surface, and openings of the plurality of holes on the first and second surfaces of the apparatus with a hydrophobic fluid; and
(d) placing the microhole apparatus with the coating of hydrophobic fluid into an environment favorable to simultaneously conducting a plurality of polynucleotide reactions on the plurality of liquid samples.
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Abstract
Disclosed herein are apparatuses and methods for conducting multiple simultaneous micro-volume chemical and biochemical reactions in an array format. In one embodiment, the format comprises an array of microholes in a substrate. Besides serving as an ordered array of sample chambers allowing the performance of multiple parallel reactions, the arrays can be used for reagent storage and transfer, library display, reagent synthesis, assembly of multiple identical reactions, dilution and desalting. Use of the arrays facilitates optical analysis of reactions, and allows optical analysis to be conducted in real time. Included within the invention are kits comprising a microhole apparatus and a reaction component of the method(s) to be carried out in the apparatus.
294 Citations
18 Claims
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1. A method for simultaneously conducting a plurality of polynucleotide reactions, the method comprising:
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(a) providing a microhole apparatus comprising a substrate having a first surface and a second surface and a plurality of holes extending through the substrate from the first surface to the second surface; (b) introducing a plurality of liquid samples comprising a nucleic acid into the plurality of holes; (c) coating the first surface, the second surface, and openings of the plurality of holes on the first and second surfaces of the apparatus with a hydrophobic fluid; and (d) placing the microhole apparatus with the coating of hydrophobic fluid into an environment favorable to simultaneously conducting a plurality of polynucleotide reactions on the plurality of liquid samples. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification