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Compositions and methods for producing isoprene free of C5 hydrocarbons under decoupling conditions and/or safe operating ranges

  • US 8,906,658 B2
  • Filed: 01/14/2013
  • Issued: 12/09/2014
  • Est. Priority Date: 07/02/2008
  • Status: Active Grant
First Claim
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1. A method of producing isoprene, the method comprising:

  • (a) culturing recombinant cells comprising a heterologous isoprene synthase polypeptide for the production of isoprene in a gas phase; and

    (b) producing isoprene in the gas phase, wherein the gas phase comprises a nonflammable concentration of isoprene and greater than or about 9.5% (volume) oxygen, and wherein the cells produce greater than about 400 nmole/gwcm/hr of isoprene.

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