Compositions and methods for producing isoprene free of C5 hydrocarbons under decoupling conditions and/or safe operating ranges
First Claim
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1. A method of producing isoprene, the method comprising:
- (a) culturing recombinant cells comprising a heterologous isoprene synthase polypeptide for the production of isoprene in a gas phase; and
(b) producing isoprene in the gas phase, wherein the gas phase comprises a nonflammable concentration of isoprene and greater than or about 9.5% (volume) oxygen, and wherein the cells produce greater than about 400 nmole/gwcm/hr of isoprene.
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Abstract
The invention features methods for producing isoprene from cultured cells wherein the cells in the stationary phase. The invention also provides compositions that include these cultured cells and/or increased amount of isoprene. The invention also provides for systems that include a non-flammable concentration of isoprene in the gas phase. Additionally, the invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity.
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Citations
29 Claims
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1. A method of producing isoprene, the method comprising:
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(a) culturing recombinant cells comprising a heterologous isoprene synthase polypeptide for the production of isoprene in a gas phase; and (b) producing isoprene in the gas phase, wherein the gas phase comprises a nonflammable concentration of isoprene and greater than or about 9.5% (volume) oxygen, and wherein the cells produce greater than about 400 nmole/gwcm/hr of isoprene. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29)
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Specification