Method for the assessment of particles and a system and device for use in the method

• US 8,906,697 B2
• Filed: 06/23/2005
• Issued: 12/09/2014
• Est. Priority Date: 11/05/1998
• Status: Expired due to Term

• Current Assignee: ChemoMetec A/S
• Original Assignee: ChemoMetec A/S
• Inventors: Glensbjerg, Martin
• Primary Examiner(s): Nguyen, Bao Thuy L
• Assistant Examiner(s): Hollinden, Gary E
• Application Number:
  US11/296,662
• Publication Number:
  US 20070207551A1
• Time in Patent Office: 3,456 Days
• Field of Search: 422/81, 422 8205- 8211, 422101-103, 436/52, 436/164, 436/172, 436/177
• US Class Current: 436/172
• CPC Class Codes: B01L 2300/021 Identification, e.g. bar codes
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: G01N 15/1433 using image recognition
• CPC Class Codes: G01N 15/1434 Optical arrangements
• CPC Class Codes: G01N 15/147 the analysis being performed on a sample stream
• CPC Class Codes: Y10T 436/117497 with a continuously flowing sample or carrier stream
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

A method and a device for the assessment of at least one parameter of particles in a liquid analyte material are disclosed. The method comprises providing a device having a sample compartment with an exposing domain, an inlet through which a volume of a liquid sample representing the analyte material can been introduced, and a flow system comprising at least a channel allowing at least a portion of the volume of the liquid sample to flow within the device. The volume of the liquid sample passes into the exposing domain of the sample compartment, which can quantitatively detect spatial image data and process the detected image electromagnetic signals from the sample in the exposing domain of the device. A spatial image representation of the exposing domain, and processing the detected image presentation obtaining the assessment of the at least one parameter is generated in the device.

Claims

1. A sampling device adapted to be used in a system for the assessment of the number of particles in a liquid analyte material, the sampling device comprising

a sample compartment including an exposing domain, said exposing domain allowing electromagnetic signals from a sample in the exposing domain to pass to a detection device so as to form spatial image of the exposing domain on the detection device,

only one inlet through which a volume of a liquid sample representing the analyte material can be introduced, and having no sample outlet,

a flow system having at least a channel allowing at least a portion of the volume of the liquid sample to flow within the sampling device, wherein at least a part of the channel through which the sample flows before entering the sample compartment has a plurality of bends or obstructions resulting in substantial turbulent flow in liquid passing the bends or obstructions, and

said sampling device being configured to position said exposing domain in relation to said detection device to provide an assessment of said at least one particle parameter of the liquid analyte material,

the device having data concerning dimensions of the device which influence the volume of the exposing domain, the data being readable by the detection device or another device adapted to read the data.

2. A sampling device according to claim 1, wherein the flow system additionally comprises a compartment or a flow channel part in which at least a part of one or more reaction components initially loaded in the compartment or flow channel is added to at least a portion of the volume of the liquid sample representing the analyte material.

3. A sampling device according to claim 2, wherein at least one of the reaction components is in freeze-dried form.

4. A device according to claim 1, wherein part of the channel provides laminar flow in the liquid sample.

5. A sampling device according to claim 1, wherein the flow system has one or more means for regulating the velocity of the flow into or within the device.

6. A sampling device according to claim 1, wherein the sampling device has a filter material and can perform one or more operations on the liquid sample, the operations being selected from the group consisting of filtration, concentration and magnetic attraction.

7. A sampling device according to claim 1, containing one or more compartment(s) or domain(s) which allows spectrophotometric measurement for the determination of any chemical property, the spectrophotometric measurement being selected from the group consisting of:

mid-infrared attenuation, near- infrared attenuation, visible attenuation, ultra-violet attenuation, photoluminescence, raman scatter, and nuclear magnetic resonance.

8. A sampling device according to claim 1, wherein the interior of the sample compartment has an average depth of between 20 μ

m and 2000 μ

m.

9. A sampling device according to claim 1, wherein the sample compartment has dimensions, in a plane parallel to an exposing window, in the range between 1 mm by 1 mm and 10 mm by 10 mm.

10. A sampling device according to claim 1, wherein the volume of the exposing domain is in the range between 0.01 μ

l and 20 μ

l.

11. A sampling device according to claim 1, wherein the flow system comprises one or more mixing chambers.

12. A sampling device according to claim 1, wherein the interior of the sample compartment has an average depth of between 20 μ

m and 1000 μ

m.

13. A sampling device according to claim 1, wherein the interior of the sample compartment has an average depth of between 20 μ

m and 200 μ

m.

14. A sampling device according to claim 1, wherein the volume of exposing domain is in the range between 0.04 μ

l and 4 μ

l.

15. A sampling device according to claim 1, wherein the sampling device includes a propelling element.

16. A sampling device according to claim 1, wherein the sampling device is configured to form, in the detection device, a processable spatial image representation of the at least one particle parameter in said exposing domain.

17. A sampling device according to claim 1, wherein the sampling device is a single use device for assessing only one volume of said liquid sample introduced through said one inlet.

18. A sampling device according to claim 1, wherein the sampling device is disposable.