Methods for controlling pests using RNAi
First Claim
1. An isolated double stranded RNA molecule comprising annealed complementary strands, wherein at least one of said strands comprises a polyribonucleotide selected from the group consisting of:
- (i) polyribonucleotides complementary to at least 21 contiguous nucleotides of a target gene represented by SEQ ID NO;
2493 or SEQ ID NO;
11, or the complement thereof,(ii) polyribonucleotides complementary to at least 21 contiguous nucleotides of a target gene encoding the amino acid sequence represented by SEQ ID NO;
2494, and(iii) polyribonucleotides having at least 85% sequence identity with the polyribonucleotides of (i) or (ii),wherein ingestion of said double stranded RNA molecule inhibits expression of said target gene.
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Abstract
The present invention concerns methods for controlling insect infestation via RNAi-mediated gene silencing, whereby the intact insect cell(s) are contacted with a double-stranded RNA from outside the insect cell(s) and whereby the double-stranded RNA is taken up by the intact insect cell(s). In one particular embodiment, the methods of the invention are used to alleviate plants from insect pests. Alternatively, the methods are used for treating and/or preventing insect infestation on a substrate or a subject in need of such treatment and/or prevention. Suitable insect target genes and fragments thereof, dsRNA constructs, recombinant constructs and compositions are disclosed.
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Citations
30 Claims
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1. An isolated double stranded RNA molecule comprising annealed complementary strands, wherein at least one of said strands comprises a polyribonucleotide selected from the group consisting of:
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(i) polyribonucleotides complementary to at least 21 contiguous nucleotides of a target gene represented by SEQ ID NO;
2493 or SEQ ID NO;
11, or the complement thereof,(ii) polyribonucleotides complementary to at least 21 contiguous nucleotides of a target gene encoding the amino acid sequence represented by SEQ ID NO;
2494, and(iii) polyribonucleotides having at least 85% sequence identity with the polyribonucleotides of (i) or (ii), wherein ingestion of said double stranded RNA molecule inhibits expression of said target gene. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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Specification