Antibodies against delta-5 desaturase and uses thereof

• US 8,916,154 B2
• Filed: 01/13/2010
• Issued: 12/23/2014
• Est. Priority Date: 04/27/2009
• Status: Expired due to Fees

• Current Assignee: Abbott Laboratories Incorporated
• Original Assignee: Abbott Laboratories Incorporated
• Inventors: Das, Tapas, Pereira, Suzette L., Mukerji, Pradip, Krishnan, Padmavathy
• Primary Examiner(s): GRASER, JENNIFER E
• Application Number:
  US12/686,781
• Publication Number:
  US 20100273187A1
• Time in Patent Office: 1,805 Days
• Field of Search: 530/387.1, 530/388.1, 530/388.26, 530/388.5, 424/130.1, 424/141.1, 424/146.1, 424/152.1
• US Class Current: 424/146.1
• CPC Class Codes: C07K 16/14 against material from fungi, algea or lichens
• CPC Class Codes: C07K 16/40 against enzymes
• CPC Class Codes: C07K 2319/23 containing a GST-tag

Abstract

Disclosed are antibodies that specifically recognize Δ5-desaturase, methods of producing the antibodies, nucleotides and polypeptides for producing the antibodies, and methods of using the antibodies. The Δ5-desaturase-specific antibodies provide improved methods of detecting Δ5-desaturase in a sample.

Claims

1. An isolated antibody generated against SEQ ID NO:

2, wherein the isolated antibody specifically binds a Δ

5-desaturase comprising the polypeptide sequence of SEQ ID NO;

2 and specifically binds at least 8 contiguous amino acid residues of SEQ ID NO;

2, and wherein said Δ

5-desaturase is derived from fungus or algae.

2. The isolated antibody of claim 1 wherein the antibody specifically binds Δ

5-desaturase derived from Saprolegnia diclina or Mortierella alpina.

3. The isolated antibody of claim 1 wherein the antibody specifically binds full-length Δ

5-desaturase derived from fungus or algae.

4. The isolated antibody of claim 1 wherein the antibody is polyclonal.

5. The isolated antibody of claim 1 wherein the antibody is monoclonal.

6. The isolated antibody of claim 1 wherein the Δ

5-desaturase polypeptide fragment is derived from fungus or algae.

7. The isolated antibody of claim 6 wherein the Δ

5-desaturase polypeptide fragment is derived from Saprolegnia diclina or Mortierella alpina.

8. The isolated antibody of claim 6 wherein the antibody is polyclonal.

9. The isolated antibody of claim 6 wherein the antibody is monoclonal.

10. An isolated antibody generated against a polypeptide fragment selected from SEQ ID NO:

2 and SEQ ID NO;

4, wherein the isolated antibody specifically binds a Δ

5-desaturase, said Δ

5-desaturase comprising the polypeptide sequence of SEQ ID NO;

2, wherein the antibody does not bind Δ

6-desaturase, and wherein both said Δ

5-desaturase and said Δ

6-desaturase are derived from fungus or algae.

11. The antibody of claim 10 wherein the antibody specifically binds Δ

5-desaturase derived from Saprolegnia diclina or Mortierella alpina.

12. The antibody of claim 10 wherein the antibody specifically binds full-length Δ

5-desaturase derived from fungus or algae.

13. The antibody of claim 10 wherein the antibody is polyclonal.

14. The antibody of claim 10 wherein the antibody is monoclonal.

15. An isolated antibody generated against a polypeptide fragment selected from SEQ ID NO:

2 and SEQ ID NO;

4, wherein said isolated antibody specifically binds a Δ

5-desaturase comprising the polypeptide sequence of SEQ ID NO;

2.

16. The isolated antibody of claim 15, wherein the Δ

5-desaturase is derived from Saprolegnia diclina or Mortierella alpina.

17. The isolated antibody of claim 15, wherein the Δ

5-desaturase is derived from fungus or algae.

18. The isolated antibody of claim 15, wherein the isolated antibody does not bind Δ

6-desaturase.

19. An isolated antibody generated against a polypeptide fragment, said isolated antibody isolated from an animal after immunizing said animal with said polypeptide fragment, wherein said isolated antibody specifically binds a Δ

5-desaturase comprising the polypeptide sequence of SEQ ID NO;

2, and wherein said polypeptide fragment is selected from SEQ ID NO;

2 and SEQ ID NO;

4.