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Methods and materials using signaling probes

  • US 8,916,503 B2
  • Filed: 02/17/2005
  • Issued: 12/23/2014
  • Est. Priority Date: 02/18/2004
  • Status: Active Grant
First Claim
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1. A method of isolating a plurality of cells, wherein a subset of the cells expresses an RNA that is not expressed by another subset of the cells, comprising the steps of:

  • introducing into cells a plurality of DNAs encoding a plurality of different RNAs, wherein each DNA further encodes a nucleic acid tag sequence, and wherein at least a subset of the plurality of DNAs encodes the same nucleic acid tag sequence;

    exposing the cells to a signaling probe that produces a detectable signal upon hybridization to said same nucleic acid tag sequence; and

    isolating, in a single application, the cells that exhibit at least one selected level of fluorescence at a selected wavelength.

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