Herbicide resistance genes

• US 8,916,752 B2
• Filed: 10/08/2012
• Issued: 12/23/2014
• Est. Priority Date: 10/28/2005
• Status: Active Grant

• Current Assignee: Corteva Agriscience LLC (Corteva, Inc.)
• Original Assignee: Dow Agrosciences LLC (Corteva, Inc.)
• Inventors: Wright, Terry R., Lira, Justin M., Walsh, Terence Anthony, Merlo, Donald, Samuel, Jayakumar P., Lin, Gaofeng
• Primary Examiner(s): Kruse, David H
• Application Number:
  US13/647,081
• Publication Number:
  US 20130035233A1
• Time in Patent Office: 806 Days
• Field of Search: None
• US Class Current: 800/300
• CPC Class Codes: C12N 15/8274 for herbicide resistance
• CPC Class Codes: C12N 15/8275 Glyphosate
• CPC Class Codes: C12N 9/0069 acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
• CPC Class Codes: C12N 9/0071 acting on paired donors with incorporation of molecular oxygen (1.14)
• CPC Class Codes: C12Y 113/11 with incorporation of two atoms of oxygen (1.13.11)

Abstract

The subject invention provides novel plants that are not only resistant to 2,4-D, but also to pyridyloxyacetate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention “stacked” together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-12 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.

Claims

1. A transgenic plant cell comprising a recombinant polynucleotide that encodes a protein having an activity that catalyzes degradation of a herbicidal compound selected from the group consisting of a phenoxypropionic auxin and a phenoxyacetic auxin, wherein said protein has at least 95% amino acid sequence identity with a sequence selected from the group consisting of SEQ ID NO:

2 and SEQ ID NO;

4.

2. The plant cell of claim 1 wherein said plant cell is selected from the group consisting of soybean, cotton, Arabidopsis, tobacco and canola.

7. The cell of claim 1, wherein said protein confers tolerance to 2,4-D to said cell.

8. The plant cell of claim 1, further comprising at least one different polynucleotide that encodes a protein that confers tolerance to the plant cell to a herbicide selected from the group consisting of glyphosate, glufosinate, HPPD-inhibitors, and dicamba.

9. The plant cell of claim 8, wherein the plant cell is selected from the group consisting of soybean, cotton, or canola.

10. The plant cell of claim 8, wherein the plant cell is a soybean cell and the herbicide is selected from the group consisting of glyphosate and dicamba.

11. The plant cell of claim 8, wherein the plant cell is a soybean cell and the herbicide is selected from the group consisting of glyphosate and glufosinate.

12. The plant cell of claim 1, further comprising a second polynucleotide that encodes a gene product having an insecticidal activity.

13. The plant cell of claim 12, wherein said gene product encoded by said second polynucleotide is selected from the group consisting of Cry1F and Cry1Ac.

14. The plant cell of claim 1 further comprising an output trait.

15. The plant cell of claim 1 further comprising an input trait.

16. A plant comprising a plurality of cells of claim 1.

17. A part, progeny, seed or asexual propagate of the plant of claim 16, wherein said part, progeny, seed or propagate comprises said polynucleotide.

18. A method for selectively controlling weeds in a field, said field containing a crop, said crop comprising plant cells of claim 1, said method comprising applying an aryloxyalkanoate herbicide to at least a portion of the crop and the weeds in the field to control the weeds.

19. A method of controlling volunteer plants of a crop comprising the plant cells of claim 1, said method comprising applying to said volunteer plants a herbicide selected from the group consisting of ACCase inhibitors, HPPD inhibitors, ALS inhibitors, and dicamba.

20. The method of claim 19 wherein the herbicide is selected from sethoxydim and clethodim.

3. A transgenic plant cell comprising a recombinant polynucleotide that encodes a protein that confers tolerance on said plant cell to a pyridyloxy acetate herbicide, wherein said protein has at least 95% amino acid sequence identity with a sequence selected from the group consisting of SEQ ID NO:

2 and SEQ ID NO;

4.

4. The cell of claim 3 wherein said pyridyloxy acetate herbicide is selected from the group consisting of triclopyr and fluroxypyr.

5. A method of producing a herbicide tolerant plant, said method comprising introducing a polynucleotide into a plant cell, wherein said polynucleotide encodes a protein having an activity that catalyzes degradation of a herbicidal compound selected from the group consisting of a phenoxypropionic auxin and as phenoxyacetic auxin, wherein said protein has at least 95% amino acid sequence identity with a sequence selected from the group consisting of SEQ ID NO:

2 and SEQ ID NO;

4, and regenerating a plant from said cell.

6. The method of claim 5 further comprising selecting transformed plant tissue that produces said protein.