Reagent preparation and dispensing device

• US 8,919,390 B2
• Filed: 11/18/2010
• Issued: 12/30/2014
• Est. Priority Date: 11/18/2010
• Status: Active Grant

• Current Assignee: Biolyph, LLC
• Original Assignee: Biolyph, LLC
• Inventors: Pearcy, Timothy, Skakoon, James G.
• Primary Examiner(s): ARNETT, NICOLAS ALLEN
• Application Number:
  US13/988,279
• Publication Number:
  US 20140048556A1
• Time in Patent Office: 1,503 Days
• Field of Search: 141/2, 141/4, 141/21, 141 25- 27, 141/392, 422501-514
• US Class Current: 141/27
• CPC Class Codes: A61M 2005/1787 Syringes for sequential delivery of fluids, e.g. first medicament and then flushing liquid
• CPC Class Codes: A61M 2005/3104 Caps for syringes without needle
• CPC Class Codes: A61M 2005/312 comprising sealing means, e.g. severable caps, to be removed prior to injection by, e.g. tearing or twisting
• CPC Class Codes: A61M 2005/3123 having air entrapping or venting means, e.g. purging channels in pistons
• CPC Class Codes: A61M 5/284 comprising means for injection of two or more media, e.g. by mixing
• CPC Class Codes: A61M 5/285 with sealing means to be broken or opened
• CPC Class Codes: A61M 5/31511 Piston or piston-rod constructions, e.g. connection of piston with piston-rod A61M5/5066 takes precedence
• CPC Class Codes: B01L 3/0206 of the plunger pump type
• CPC Class Codes: B01L 3/52 Containers specially adapted for storing or dispensing a reagent B01L3/02 takes precedence; containers for medical or pharmaceutical purposes A61J1/00; containers in general B65D; storing or dispensing test elements G01N33/4875; automated reagent dispensing G01N35/1002

Abstract

A reagent preparation and dispensing device includes a body having a reaction chamber for a reagent and a solution reservoir for a solution. The solution reservoir is isolated from the reaction chamber. A plunger is movably coupled with the body. Movement of the plunger from a starting position to a seated position pushes the solution into the reaction chamber. A dispensing path extends from the reaction chamber out of the body. The dispensing path is configured to dispense a reagent mixture formed from the solution and the reagent. A vent path extends from the reaction chamber, the vent path vents reaction chamber gas displaced by the addition of the solution to the reaction chamber throughout movement of the plunger from the starting position to the seated position.

Claims

1. An apparatus comprising:

a body including;

a reaction chamber including a reagent, and

a solution reservoir including a solution, the solution reservoir is isolated from the reaction chamber;

a plunger movably coupled with the body, movement of the plunger from a starting position to a seated position pushes the solution into the reaction chamber;

a dispensing path extending from the reaction chamber and out of the body, the dispensing path is configured to dispense a reagent mixture formed from the solution and the reagent; and

a vent path extending from the reaction chamber, the vent path vents reaction chamber gas displaced by the addition of the solution to the reaction chamber throughout movement of the plunger from the starting position to the seated position; and

a flushing chamber in the body, the flushing chamber is filled with a flushing fluid operable to push the specified amount of the reagent mixture through the dispensing path.

2. The apparatus of claim 1 further comprising a barrel movably coupled with the body, and the plunger is movably coupled with the barrel.

3. The apparatus of claim 2, wherein the vent path extends through the barrel and along the plunger.

4. The apparatus of claim 3, wherein the vent path extends along a barrel exterior through a first vent portion, and the vent path extends along a barrel interior through a second vent portion.

5. The apparatus of claim 1, wherein a semi-permeable media is disposed in the vent path.

6. The apparatus of claim 1, wherein the vent path extends through the flushing chamber.

7. The apparatus of claim 6, wherein a second plunger is operable to close the vent path, and the second plunger is operable to push the flushing fluid through the closed vent path into the reaction chamber.

8. The apparatus of claim 1, wherein the apparatus is configured to form a specified volume of reagent mixture from about 10 to 100 micro liters with 10 percent or better precision.

9. The apparatus of claim 8, wherein the apparatus is configured to dispense around at least 80 percent of the specified volume of the reagent mixture formed in the reaction chamber.

10. An apparatus comprising:

a body including;

a reaction chamber including a reagent, and

a solution reservoir including a solution, the solution reservoir is isolated from the reaction chamber;

a plunger movably coupled with the body, movement of the plunger pushes the solution into the reaction chamber;

a dispensing path extending from the reaction chamber and out of the body, the dispensing path is configured to deliver a reagent mixture formed from the solution and the reagent;

a flushing chamber including a flushing fluid in communication with the reaction chamber; and

a vent path extending from the reaction chamber, the vent path includes open and closed configurations;

in the open configuration, the vent path extend outside of the body, and the vent path vents reaction chamber gas displaced by the addition of the solution to the reaction chamber, and

in the closed configuration, the vent path is closed, and flushing fluid delivered from the flushing chamber dispenses the reagent mixture through the dispensing path.

11. The apparatus of claim 10 further comprising a barrel movably coupled with the body, and the plunger is movably coupled with the barrel.

12. The apparatus of claim 11, wherein the vent path extends through the barrel and along the plunger.

13. The apparatus of claim 10, wherein the vent path extends along a barrel exterior through a first vent portion, and the vent path extends along a barrel interior through a second vent portion.

14. The apparatus of claim 10, wherein a semi-permeable media is disposed in the vent path.

15. The apparatus of claim 10, wherein the vent path extends through the flushing chamber.

16. The apparatus of claim 15, wherein a second plunger is operable to close the vent path, and the second plunger is operable to push the flushing fluid through the closed vent path into the reaction chamber.

17. The apparatus of claim 10, wherein the apparatus is configured to form a specified volume of reagent mixture from about 10 to 200 micro liters with 10 percent or better precision.

18. The apparatus of claim 17, wherein the apparatus is configured to dispense at least around 90 percent of the specified volume of the reagent mixture formed in the reaction chamber.

19. An apparatus comprising:

a body including;

a reaction chamber including a reagent, and

a solution reservoir including a solution, the solution reservoir is isolated from the reaction chamber;

a first plunger movably coupled with the body, movement of the first plunger pushes the solution into the reaction chamber with the reagent to form a reagent mixture;

a second plunger movably coupled with the body, movement of the second plunger dispenses a specified amount of the reagent mixture from the body; and

a vent path in communication with the reaction chamber, the vent path includes open and closed configurations;

in the open configuration, the vent path extends from the reaction chamber to outside of the body, and the vent path vents gas displaced from the reaction chamber by the addition of the solution to the reaction chamber, and

in the closed configuration, the vent path is closed by movement of the second plunger; and

a flushing chamber in the body containing a flushing fluid.

20. The apparatus of claim 19, wherein the flushing chamber is in communication with the vent path and the reaction chamber, and the second plunger is operable to push flushing fluid into the reaction chamber through the vent path in the closed configuration.

21. The apparatus of claim 19, wherein the flushing chamber is formed by the second plunger and a vent wall.

22. The apparatus of claim 19, wherein a volume of the flushing chamber is greater than a volume of the reaction chamber.

23. The apparatus of claim 19, wherein the vent path extends from the reaction chamber between the body and a barrel movably coupled with the body.

24. A method of making a reagent preparation device comprising:

positioning a solution within a body;

positioning a reagent within a reaction chamber in the body, the reagent is isolated from the solution;

movably coupling an activator with the body, the activator is movable to force the solution into the reaction chamber and form a reagent mixture when the activator is moved from a starting position to a seated position;

forming a closed dispensing tip extending from the reaction chamber and out of the body;

forming a vent path extending from the reaction chamber to outside of the body, the vent path vents gas from the reaction chamber displaced by the addition of the solution to the reaction chamber, and the vent path vents gas throughout movement of the activator from the starting position to the seated position; and

forming a flushing chamber within the body, and the flushing chamber is in fluid communication with the reaction chamber through the vent path.

25. The method of claim 24, wherein movably coupling the activator with the body includes movably coupling a plunger with the body.

26. The method of claim 24 further comprising movably coupling a barrel with the body.

27. The method of claim 26, wherein forming the vent path includes extending the vent path between the barrel and the body.

28. The method of claim 24, wherein forming the vent path includes forming the vent path through the body from the reaction chamber, and forming the closed dispensing tip includes forming the closed dispensing tip extending from the reaction chamber in an opposed direction to the vent path.

29. The method of claim 24 further comprising movably coupling a second plunger with the body, and the second plunger is operable to close the vent path and push a flushing fluid through the vent path into the reaction chamber.

30. The method of claim 24, wherein forming the closed dispensing tip includes extending the closed dispensing tip from a first portion of the reaction chamber, and forming the vent path includes extending the vent path from a second portion of the reaction chamber opposed to the first portion.

31. A method of reagent preparation and dispensing comprising:

opening a sealed reaction chamber within a body, the reaction chamber containing a reagent;

reconstituting the reagent with a solution retained within the body to form a reagent mixture, reconstituting including adding the solution to the reaction chamber;

venting displaced gas from the reaction chamber, the gas is displaced by addition of the solution, the vented gas passing through a vent path extending through the body away from a dispensing tip; and

dispensing a specified amount of the reagent mixture, dispensing including closing the vent path and pushing a flushing fluid through the vent path into the reaction chamber.

32. The method of claim 31, wherein venting gas includes preventing pressurization within the reaction chamber during reconstitution.

33. The method of claim 31, wherein dispensing the specified amount of the reagent mixture includes pressurizing the reaction chamber.

34. The method of claim 31, wherein venting gas includes venting gas through the body in a direction opposed to a direction of dispensing the specified amount of the reagent mixture.

35. The method of claim 31, wherein reconstituting of the reagent is adjacent to the dispensing tip in a first portion of the reaction chamber, and the gas is vented from a second portion of the reaction chamber remote from the first portion.

36. The method of claim 31, wherein reconstituting and dispensing of the specified amount of the reagent mixture are performed with the body in substantially the same orientation.

37. The method of claim 31, wherein dispensing the specified amount of the reagent mixture includes moving a plunger relative to the body, and closing the vent path includes engaging the plunger with a vent wall to seal a flushing chamber formed by the plunger and the vent wall, and pushing the flushing fluid through the vent path includes moving the plunger through the flushing chamber.