Method for purifying urate oxidase tetramers and octamers
First Claim
1. A method for purifying urate oxidase (uricase) tetramers and octamers, the method comprising(a) separating a uricase solution comprising uricase aggregates larger than octamers, uricase tetramers, and uricase octamers into fractions,(b) detecting said aggregates larger than octamers in said fractions by measuring light scattering;
- and(c) excluding fraction(s) containing said aggregates larger than octamers as detected in (b), thereby producing purified uricase tetramers and octamers.
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Abstract
A naturally occurring or recombinant protein, especially a mutein of porcine urate oxidase (uricase), that is essentially free of large aggregates can be rendered substantially non-immunogenic by conjugation with a sufficiently small number of strands of polymer such that the bioactivity of the protein is essentially retained in the conjugate. Such conjugates are unusually well suited for treatment of chronic conditions because they are less likely to induce the formation of antibodies and/or accelerated clearance than are similar conjugates prepared from protein preparations containing traces of large aggregates.
57 Citations
3 Claims
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1. A method for purifying urate oxidase (uricase) tetramers and octamers, the method comprising
(a) separating a uricase solution comprising uricase aggregates larger than octamers, uricase tetramers, and uricase octamers into fractions, (b) detecting said aggregates larger than octamers in said fractions by measuring light scattering; - and
(c) excluding fraction(s) containing said aggregates larger than octamers as detected in (b), thereby producing purified uricase tetramers and octamers. - View Dependent Claims (2, 3)
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Specification