Reagent composition for electrochemical biosensors
First Claim
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1. A reagent composition for making electrochemical biosensors comprising:
- (a) glucose dehydrogenase (GDH) and co-factor pyrrolo-quinoline quinone (PQQ) for oxidizing glucose in a biological sample;
(b) 2 to about 10 wt % of a hydrophilic polymer selected from cellulose derivatives, based on the total weight of the composition;
(c) about 1 to about 6 wt % of amorphous untreated silica powder, based on the total weight of the composition;
(d) a buffer sufficient to maintain pH in the range of from about 4.5 to about 6.5;
(e) a surfactant; and
(f) a mediator.
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Abstract
A reagent composition containing GDH-PQQ as an enzyme-co-factor and screen-printed on working and counter electrodes of electrochemical biosensors, maintains activity of the enzyme reagents by proper selection of components. A preferred composition includes hydrophilic polymers, amorphous untreated silica, buffers, surfactants, and a mediator. For example, the biosensor is useful in the amperometric determination of glucose.
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Citations
28 Claims
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1. A reagent composition for making electrochemical biosensors comprising:
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(a) glucose dehydrogenase (GDH) and co-factor pyrrolo-quinoline quinone (PQQ) for oxidizing glucose in a biological sample; (b) 2 to about 10 wt % of a hydrophilic polymer selected from cellulose derivatives, based on the total weight of the composition; (c) about 1 to about 6 wt % of amorphous untreated silica powder, based on the total weight of the composition; (d) a buffer sufficient to maintain pH in the range of from about 4.5 to about 6.5; (e) a surfactant; and (f) a mediator. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A reagent composition for making electrochemical biosensors comprising:
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(a) glucose dehydrogenase (GDH) and co-factor pyrrolo-quinoline quinone (PQQ) for oxidizing glucose in a biological sample, wherein about 1-8 units of said GDH-PQQ is present for each milligram of the total weight of the composition; (b) 2 to about 10 wt % of a hydrophilic polymer selected from cellulose derivatives, based on the total weight of the composition; (c) about 1 to about 6 wt % of a thickening agent selected from the group consisting of amorphous untreated silica powder, talc, mica, diatomaceous earth, and natural and modified clays, based on the total weight of the composition; (d) about 30 to about 200 mM of a buffer sufficient to maintain pH in the range of from about 4.5 to about 6.5; (e) up to about 0.5 wt % of a surfactant, based on the total weight of the composition; and (f) about 10 to about 20 wt % of a mediator, based on the total weight of the composition. - View Dependent Claims (19, 20, 21)
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22. A reagent composition for making electrochemical biosensors comprising:
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(a) glucose dehydrogenase (GDH) and co-factor pyrrolo-quinoline quinone (PQQ) for oxidizing glucose in a biological sample; (b) 2 to about 10 wt % of a hydroxyethyl cellulose polymer; (c) amorphous untreated silica powder; (d) a calcium acetate buffer sufficient to maintain pH in the range of from about 4.5 to about 6.5; (e) an alkyl aryl polyether alcohol surfactant; and (f) a potassium ferricyanide mediator. - View Dependent Claims (23, 24, 25)
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26. A reagent composition for making electrochemical biosensors comprising:
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(a) an enzyme for oxidizing glucose in a biological sample; (b) 2 to about 10 wt % of a hydrophilic polymer selected from cellulose derivatives, based on the total weight of the composition; (c) about 1 to about 6 wt % of a thickening agent selected from the group consisting of amorphous untreated silica powder, talc, mica, diatomaceous earth, and natural and modified clays, based on the total weight of the composition; (d) a buffer sufficient to maintain pH in the range of from about 4.5 to about 6.5; (e) a surfactant; and (f) a mediator. - View Dependent Claims (27, 28)
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Specification