Devices and systems for creation of DNA cluster arrays
First Claim
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1. A system for creation of samples in a flow cell, the system comprising:
- a system chassis and a flow cell placement area that is supported by the system chassis, the placement area being configured to receive the flow cell;
at least one manifold having a manifold body configured to be placed onto the flow cell;
at least one reagent reservoir;
at least one temperature control component; and
at least one computer control component;
wherein the reagent reservoir, the manifold, the temperature control component, and the computer control component are functionally connected to one another;
wherein the reagent reservoir and the manifold are capable of being directly or indirectly fluidly connected to one another when connected to said flow cell;
wherein the reagent reservoir, the manifold body, and the flow cell are arranged such that one or more fluids are capable of flow under control of the computer control component from the reagent reservoir, through the manifold body, and into said flow cell, allowing creation of the sample;
wherein the manifold body includes a manifold port that is configured to removably engage a flow cell port of the flow cell to fluidly connect the reagent reservoir to the flow cell, the manifold body configured to move with respect to the placement area to removably engage the manifold port and flow cell port; and
wherein the system further comprises a flow cell holder that is configured to move between open and closed positions to permit access to the flow cell placement area for placing the flow cell on the flow cell placement area and for placing the manifold body onto the flow cell, the manifold body configured to move with respect to the flow cell holder when the flow cell holder is stationary in the open position, the flow cell holder pressing the manifold body against the flow cell when in the closed position so that the manifold body presses the flow cell toward the flow cell placement area.
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Abstract
The present invention comprises systems and devices for isothermal amplification of polynucleotide sequences to produce DNA cluster arrays.
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Citations
20 Claims
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1. A system for creation of samples in a flow cell, the system comprising:
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a system chassis and a flow cell placement area that is supported by the system chassis, the placement area being configured to receive the flow cell; at least one manifold having a manifold body configured to be placed onto the flow cell; at least one reagent reservoir;
at least one temperature control component; and
at least one computer control component;wherein the reagent reservoir, the manifold, the temperature control component, and the computer control component are functionally connected to one another; wherein the reagent reservoir and the manifold are capable of being directly or indirectly fluidly connected to one another when connected to said flow cell; wherein the reagent reservoir, the manifold body, and the flow cell are arranged such that one or more fluids are capable of flow under control of the computer control component from the reagent reservoir, through the manifold body, and into said flow cell, allowing creation of the sample; wherein the manifold body includes a manifold port that is configured to removably engage a flow cell port of the flow cell to fluidly connect the reagent reservoir to the flow cell, the manifold body configured to move with respect to the placement area to removably engage the manifold port and flow cell port; and wherein the system further comprises a flow cell holder that is configured to move between open and closed positions to permit access to the flow cell placement area for placing the flow cell on the flow cell placement area and for placing the manifold body onto the flow cell, the manifold body configured to move with respect to the flow cell holder when the flow cell holder is stationary in the open position, the flow cell holder pressing the manifold body against the flow cell when in the closed position so that the manifold body presses the flow cell toward the flow cell placement area. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. A method of creating nucleic acid cluster arrays through isothermal nucleic acid amplification, the method comprising:
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a.) providing a cluster station system, the system comprising at least one manifold having a manifold body;
at least one reagent reservoir;
at least one temperature control component; and
at least one computer control component, the system also including a system chassis and a flow cell placement area that is supported by the system chassis, the placement area being configured to receive a flow cell, the manifold body configured to be placed onto the flow cell;wherein the reagent reservoir, the manifold, the temperature control component, and the computer control component are functionally connected to one another; wherein the reagent reservoir and the manifold body are directly or indirectly fluidly connected to one another when connected to a flow cell; wherein the reagent reservoir, the manifold body, and the flow cell are arranged such that one or more fluids are capable of flow under control of the computer control component from the reagent reservoir, through the manifold body, and onto a surface of the flow cell; and wherein the manifold body includes a manifold port that is configured to removably engage a flow cell port of the flow cell to fluidly connect the reagent reservoir to the flow cell, the manifold body movable with respect to the placement area to removably engage the manifold port and flow cell port; wherein the system further comprises a flow cell holder that is configured to move between open and closed positions to permit access to the flow cell placement area for placing the flow cell on the flow cell placement area and for placing the manifold body onto the flow cell, the manifold body configured to move with respect to the flow cell holder when the flow cell holder is stationary in the open position, the flow cell holder pressing the manifold body against the flow cell when in the closed position so that the manifold body presses the flow cell toward the flow cell placement area; b.) providing amplification reagents, a chemical denaturant, and template nucleic acid; and c.) flowing cycles of the amplification reagents and denaturant under control of the cluster station device through the flow cell, the flow cell comprising the nucleic acid template, wherein the flow cell is kept under substantially isothermal conditions by the cluster station system, and wherein the nucleic acid template is amplified within the flow cell thus creating nucleic acid cluster arrays.
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Specification