Paper-based microfluidic systems

• US 8,921,118 B2
• Filed: 03/27/2009
• Issued: 12/30/2014
• Est. Priority Date: 03/27/2008
• Status: Active Grant

• Current Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Original Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Inventors: Siegel, Adam C., Phillips, Scott T., Dickey, Michael D., Rozkiewicz, Dorota, Wiley, Benjamin, Whitesides, George M., Martinez, Andres W.
• Primary Examiner(s): HIXSON, CHRISTOPHER
• Application Number:
  US12/934,857
• Publication Number:
  US 20110111517A1
• Time in Patent Office: 2,104 Days
• US Class Current: 436/164
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0642 Filling fluids into wells by specific techniques
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/025 Displaying results or values with integrated means
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0809 rectangular shaped
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2300/12 Specific details about materials
• CPC Class Codes: B01L 2300/126 Paper
• CPC Class Codes: B01L 2300/161 Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: Y10T 436/25 including sample preparation
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

Paper-based microfluidic systems and methods of making the same are described.

Claims

1. An assay device comprising a porous, hydrophilic substrate having first and second faces, a fluid-impermeable barrier permeating the thickness of the substrate and defining a boundary of an assay region and a boundary of a channel region fluidically connected to the assay region, an electrically conductive material disposed on one of the first and second faces of the substrate and spanning the channel region in proximity to said assay region, a detection reagent disposed in the assay region, and an insulating material disposed between the conductive material and the substrate.

2. The device of claim 1 wherein the electrically conductive material is a metal or conductive polymer.

3. The device of claim 2 wherein the metal is Sn, Zn, Au, Ag, Ni, Pt, Pd, Al, In, or Cu.

4. The device of claim 1 wherein the barrier defines a plurality of assay regions and a plurality of channel regions, and the device comprises one or more strips of conductive material spanning one or more said channel regions.

5. The device of claim 1 wherein the channel region is in fluid communication with a sample deposition region and provides a fluidic pathway within the substrate between the sample deposition region and the assay region.

6. The device of claim 1 comprising a pattern of said barriers comprising a photoresist or a curable polymer.

7. The device of claim 1 comprising a substrate comprising nitrocellulose acetate, cellulose acetate, cellulosic paper, filter paper, tissue paper, writing paper, paper towel, cloth, or porous polymer film.

8. The device of claim 1 wherein the conductive material comprises a strip.

9. The device of claim 1 further comprising an electric current source connected to the conductive material for inducing resistive heating therein.

10. The device of claim 9 wherein the conductive material has a resistance of about 20Ω

to about 500Ω

.

11. The device of claim 1 wherein the conductive material functions as a valve to modulate flow of fluid through said channel region.

12. The device of claim 1 further comprising an integrated circuit, resistor, capacitor, transistor, diode, or a mechanical switch attached to the channel region or to the conductive material.

13. The device of claim 1 wherein the detection reagent responds to the presence of an analyte to produce a signal visible to the naked eye.

14. The device of claim 1 wherein said conductive material is adapted for pumping a fluid, evaporating a fluid, concentrating an analyte by evaporation, controlling the direction of flow of a fluid, turning on/off a flow of a fluid, sensing temperature of a fluid in said substrate, heating a fluid for reaction or incubation of cells, cooling a fluid in said substrate, temperature cycling for executing PCR in said substrate, concentrating a magnetic field in said substrate, applying a magnetic field for separations, capturing particles or analytes, or applying an electrical or magnetic field in said substrate for mixing.

15. A method of performing an assay comprising providing an assay device of claim 1, applying an electric current to the conductive material, contacting the channel region with a fluid sample, and observing a visually detectable signal in the assay region.

16. A method of controlling the movement of a fluid sample through an assay device, the method comprising providing the assay device of claim 1, contacting the channel region with a fluid sample, and applying an electric current to the conductive material thereby to modulate fluid flow of the sample in the channel region.

17. The device of claim 7, wherein the electrically conductive material spans the channel region at about 0.5 mm from the assay region.

18. The device of claim 17, wherein the device is configured so that heating the channel region in proximity to the electrically conductive material to a temperature greater than 60°

C. prevents water from wicking through the channel past the electrically conductive material.

19. The device of claim 18, wherein the device is configured so a portion of the channel heated to 65-75°

C. cools to 23°

C. in less than five seconds upon termination of electrical current to the electrically conductive material.

20. The device of claim 17, wherein the electrically conductive material is a gold wire, and the insulating material is tape.