plants with high levels of anticarcinogenic glucosinolates

• US 8,921,644 B2
• Filed: 04/13/2004
• Issued: 12/30/2014
• Est. Priority Date: 04/14/2003
• Status: Active Grant

• Current Assignee: Bejo Zaden BV
• Original Assignee: Bejo Zaden BV
• Inventors: Barten, Piet
• Primary Examiner(s): MCELWAIN, ELIZABETH F
• Application Number:
  US10/553,301
• Publication Number:
  US 20070033675A1
• Time in Patent Office: 3,913 Days
• Field of Search: None
• US Class Current: 800/260
• CPC Class Codes: A01H 1/101 involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine or caffeine

Abstract

A method is for providing plants belonging to the Brassicaceae family with elevated levels of anticarcinogenic glucosinolates. The plants are obtained by 1) the production of a Brassica oleracea plant with elevated levels of anticarcinogenic glucosinolates in the edible parts and 2) the use of the Brassica oleracea plant produced under 1) as a starting material for breeding Brassica varieties with elevated levels of anticarcinogenic glucosinolates.

Claims

1. A method for providing Brassica varieties with elevated levels of anticarcinogenic glucosinolates, comprising:

a) crossing a cultivated Brassica oleracea plant with a second Brassica oleracea plant; and

b) identifying a progeny Brassica oleracea plant that has at least 100 micromol gluboiberin (3-methylsulphinylpropyl glucosinolate (3MSPG)) per 100 gram of fresh weight of the edible part,

wherein the cultivated Brassica oleracea plant is sprouting broccoli (Brassica oleracea convar. botrytis var. asparagoides) variety Bordeaux or savoy cabbage (Brassica oleracea convar. capitata var. sabauda) variety Wirosa,

wherein, in the cultivated Brassica oleracea plant, the anticarcinogenic glucosinolates include at least 3MSPG and glucoraphanin (4-methylsulphinylbutyl glucosinolate (4MSBG)), and

wherein, in the cultivated Brassica oleracea plant, the concentration of 3MSPG per 100 gram of fresh weight of the edible part is greater than 280 micromol.

2. The method according to claim 1, wherein the concentration of 3MSPG per 100 gram of fresh weight of the edible part is greater than 390 micromol.

3. The method according to claim 1, wherein the concentration of 3MSPG per 100 gram of fresh weight of the edible part is greater than 790 micromol.

4. The method according to claim 1, wherein the concentration of 4MSBG per 100 gram of fresh weight of the edible part is greater than 140 micromol.

5. The method according to claim 1, wherein the cultivated Brassica oleracea plant is sprouting broccoli (Brassica oleracea convar. botrytis var. asparagoides) variety Bordeaux.

6. The method according to claim 1, wherein the cultivated Brassica oleracea plant is Savoy cabbage (Brassica oleracea convar. capitata var. sabauda) variety Wirosa.

7. The method according to claim 1, wherein the second Brassica oleracea plant is selected from the group consisting of sprouting broccoli (Brassica oleracea convar. botrytis var. asparagoides) of variety Bordeaux;

and Savoy cabbage (Brassica oleracea convar. capitata var. sabauda) of variety Wirosa.

8. A method for providing Brassica varieties with an elevated level of glucoiberin (3-methylsulphinylpropyl glucosinolate (3MSPG)), comprising:

a) crossing a cultivated Brassica oleracea plant with a second Brassica oleracea plant, wherein the cultivated Brassica oleracea plant is sprouting broccoli (Brassica oleracea convar. botrytis var. asparagoides) variety Bordeaux or savoy cabbage (Brassica oleracea convar. capitata var. sabauda) variety Wirosa; and

b) identifying a progeny Brassica oleracea plant that has at least 100 micromol 3MSPG per 100 gram of fresh weight of the edible part.