Cells for biochemical analysis, kit for biochemical analysis, and biochemical analyzer

• US 8,922,765 B2
• Filed: 08/07/2013
• Issued: 12/30/2014
• Est. Priority Date: 03/07/2006
• Status: Expired due to Fees

• Current Assignee: Hitachi, Ltd.
• Original Assignee: Hitachi, Ltd.
• Inventors: Fujimura, Toru, Kuwabara, Kosuke, Ide, Tatsuro
• Primary Examiner(s): NGUYEN, SANG H
• Application Number:
  US13/961,173
• Publication Number:
  US 20130315784A1
• Time in Patent Office: 510 Days
• Field of Search: 356/244, 356/246, 356 39- 41, 422/56, 422/61, 422/100, 422/82.05, 422/82.09, 422/69, 250/573, 250/216, 250/574, 435/287.2, 435/288.3, 435/293.1, 435/420, 435/4, 435/6
• US Class Current: 356/244
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/31 Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry G01N21/72 takes precedence
• CPC Class Codes: G01N 21/55 Specular reflectivity
• CPC Class Codes: G01N 2201/061 Sources
• CPC Class Codes: G01N 2201/08 Optical fibres; light guides
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

The invention makes it possible to measure binding of a biochemical substance with a high throughput and with high sensitivity using a small cell capable of being filled with a small amount of chemical solution. A space between a first substrate and a second substrate such that probes are immobilized on their mutually facing planes is used as a cell that houses a specimen solution. Light is irradiated from a first substrate side, and reflected light is subjected to spectroscopy. Binding of the target with the probe is detected by a wavelength shift in the refection spectrum.

Claims

1. A cell for biochemical analysis, comprising:

a first member having a silicon substrate and an optical thin film on the silicon substrate, a first site of a surface of the optical thin film having a biochemical substance immobilized thereon; and

a second member, a second site of a surface of the second member having the same biochemical substance as the biochemical substance immobilized on the first site of the surface of the optical thin film immobilized thereon;

wherein the first site of the surface of the optical thin film and the second site of the surface of the second member face each other to form a gap for introducing an aqueous solution into the gap.

2. The cell for biochemical analysis according to claim 1, wherein same kind of linkers are on the surface of the optical thin film and the surface of the second member.

3. The cell for biochemical analysis according to claim 2, wherein the linkers are made of silane coupling agents.

4. The cell for biochemical analysis according to claim 1, wherein the optical thin film is a silicon nitride film.