Motion control systems and methods for biosensor scanning

• US 8,922,860 B2
• Filed: 09/13/2012
• Issued: 12/30/2014
• Est. Priority Date: 02/22/2011
• Status: Expired due to Fees

• Current Assignee: Corning Incorporated
• Original Assignee: Corning Incorporated
• Inventors: Tovey, Cameron John
• Primary Examiner(s): Doak, Jennifer L.
• Application Number:
  US13/613,141
• Publication Number:
  US 20130063724A1
• Time in Patent Office: 838 Days
• US Class Current: 359/199.1
• CPC Class Codes: G01N 2021/7773 Reflection
• CPC Class Codes: G01N 2021/7793 Sensor comprising plural indicators
• CPC Class Codes: G01N 2021/7796 Special mountings, packaging of indicators
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/774 the reagent being on a grating or periodic structure
• CPC Class Codes: G01N 2201/10 Scanning
• CPC Class Codes: G01N 2201/1042 X, Y scan, i.e. object moving in X, beam in Y
• CPC Class Codes: G01N 2201/105 Purely optical scan

Abstract

Motion control system and method for biosensor scanning that include inputting to a multi-axis motion controller move commands associated with the scan path as defined by multiple axes. The multiple axes including an x-baseline coordinate x₀, a y-baseline coordinate y₀, an x-direction oscillation amplitude x₁, a y-direction oscillation amplitude y₁, an oscillation frequency f and a phase φ. The multi-axis motion controller outputs digital commanded positions for each of the multiple axes. A post-processor receives the commanded positions and generates parameterized commanded positions x and y that each include a baseline motion component and an oscillating motion component. The parameterized commanded positions cause the scanning optical system to deflect the light beam to scan the beam spot over the scan path to scan the biosensor.

Claims

1. A method of defining a scan path using a scanning optical system to scan a beam spot formed by a light beam over one or more biosensors supported by a microplate, comprising:

inputting to a multi-axis motion controller move commands associated with the scan path as defined by multiple axis comprising an x-baseline coordinate x₀, a y-baseline coordinate y₀, an x-direction oscillation amplitude x₁, a y-direction oscillation amplitude y₁, an oscillation frequency f and a phase φ

;

outputting from the multi-axis motion controller commanded positions for each of the multiple axes;

receiving the commanded positions with a post-processor and generating therein parameterized commanded positions x and y defined the following equations where t is time and OSC is an oscillatory function;

x=x₀+x₁·

OSC(2π

·

f·

t+φ

) and y=y₀+y₁·

OSC(2π

·

f·

t+φ

); and

inputting the parameterized commanded positions x and y to a scanning optical system to cause the scanning optical system to deflect the light beam to scan the beam spot over the scan path.

2. The method of claim 1, wherein the oscillatory function OSC includes at least one function selected from the group of functions comprising:

a sine function, a cosine function, a square wave, a triangular wave, a periodic wave made up of polynomial segments, a periodic wave made up of hyperbolic segments, a sigmoid function, and a periodic function filtered to remove select resonant frequencies of the scanning optical system.

3. The method of claim 1, wherein the parameterized commanded positions x and y are digital, and further comprising;

converting the digital parameterized commanded positions x and y to parameterized drive output positions x and y; and

amplifying the parameterized drive output positions x and y and inputting same into the scanning optical system.

4. The method of claim 3, further comprising generating the amplified commanded positions x and y as respective x and y currents.

5. The method of claim 1, wherein the scanning optical system includes one of a scanning galvanometer, a flexure-based scanning mirror, a micro-electro-mechanical system (MEMS) mirror, an oscillating plane mirror, a rotating multifaceted mirror, and a piezo-electric-driven mirror.

6. The method of claim 1, further comprising:

defining one of the move command as an amount of time to move the light spot over at least a portion of the scan path; and

defining the following move commands for each axis;

a start value, an end value, a velocity limit, an acceleration limit, a start velocity, and an end velocity.

7. The method of claim 1, further comprising inputting the move commands to the multi-axis motion controller from a system controller.

8. The method of claim 1, wherein the scan path has a velocity and an oscillation amplitude, and further comprising smoothly reducing the oscillation amplitude to zero as the scan path velocity decreases to a threshold value.

9. The method of claim 8, further comprising employing a sigmoid function in smoothly reducing the oscillation amplitude.

10. The method of claim 1, wherein the scan path includes a baseline component, and wherein baseline component includes sections of the scan path between adjacent biosensors that have a greater velocity than baseline component sections of the scan path that scan over the biosensors.

11. A system for defining a scan path for a beam spot formed by a light beam over a microplate that operably supports at least one biosensor, comprising:

a multi-axis motion controller configured to receive move commands associated with the scan path as defined by respective axes, the axes including an x-baseline coordinate x₀, a y-baseline coordinate y₀, an x-direction oscillation amplitude x₁, a y-direction oscillation amplitude y₁, an oscillation frequency f and a phase φ

, and output commanded positions for each of the multiple axes;

a post-processor configured to receive the commanded positions and generate therefrom parameterized commanded positions x and y defined by the following equations where t is time and OSC is an oscillatory function;

x=x₀+x₁·

OSC(2π

·

f·

t+φ

) and y=y₀+y₁·

OSC(2π

·

f·

t+φ

); and

a scanning optical system configured to receive the parameterized commanded positions and in response thereto deflect the light beam to scan the beam spot over the scan path.

12. The system of claim 11, wherein the oscillatory function OSC includes at least one function selected from the group of functions comprising:

a sine function, a cosine function, a square wave, a triangular wave, a periodic wave made up of polynomial segments, a periodic wave made up of hyperbolic segments, a sigmoid function, and a periodic function filtered to remove select resonant frequencies of the scanning optical system.

13. The system of claim 11, further comprising first and second digital amplifiers operably connected to the post-processor and respectively configured to convert the digital parameterized commanded positions x and y to parameterized drive output positions x and y and to amplify the parameterized drive output positions x and y.

14. The system of claim 13, further comprising generating the amplified commanded positions x and y as respective x and y currents.

15. The system of claim 11, wherein the scanning optical system includes one of a scanning galvanometer, a flexure-based scanning mirror, a micro-electro-mechanical system (MEMS) mirror, an oscillating plane mirror, a rotating multifaceted mirror, and a piezo-electric-driven mirror.

16. The system of claim 11, further comprising:

a system controller operably connected to the multi-axis controller and configured to input the move commands to the multi-axis controller.

17. The system of claim 11, wherein the scan path has a velocity and an oscillation amplitude, and further comprising the post-processor being configured to smoothly reduce the oscillation amplitude to zero as the scan path velocity decreases to a threshold value.

18. The system of claim 17, further comprising the post-processor configured to employ a sigmoid function to smoothly reduce the oscillation amplitude.

19. The system of claim 18, further comprising an intermediate processor operably arranged between the multi-axis motion controller and the post-processor, the intermediate processor configured to express the parameterized commanded positions x and y in polar coordinates.

20. A method of defining a scan path for a beam spot formed by a scanning optical system, comprising:

inputting to a multi-axis motion controller move commands associated with the scan path as defined multiple axes, the multiple axes including an x-baseline coordinate x₀, a y-baseline coordinate y₀, an x-direction oscillation amplitude x₁, a y-direction oscillation amplitude y₁, an oscillation frequency f and a phase φ

;

outputting from the multi-axis motion controller digital commanded positions for each of the multiple axes;

receiving the digital commanded positions with a post-processor and generating therein analog or pulse-wave modulated parameterized commanded positions x and y that each include a baseline motion component and an oscillating motion component; and

inputting the analog or pulse-wave modulated parameterized commanded positions to a scanning optical system to cause the scanning optical system to deflect a light beam to scan the beam spot over the scan path.

21. The method of claim 20, wherein the analog or pulse-wave modulated parameterized commanded positions x and y are defined by the following equations where t is time:

x=x₀+x₁·

sin(2π

·

f·

t+φ

) and y=y₀+y₁·

sin(2π

·

f·

t+φ

).