Optical lens system and method for microfluidic devices
DCFirst Claim
1. A method of imaging electromagnetic radiation emitted from a device having a plurality of reaction sites, the method comprising:
- thermally cycling the plurality of reaction sites of the device with a thermal controller;
illuminating the device with electromagnetic radiation with an illumination system;
transmitting the electromagnetic radiation along an optical path and through an optical filter device and a multi-element optical system coupled to the optical path, wherein the multi-element optical system comprises at least five optical elements;
reducing chromatic aberration of light along the optical path;
capturing an image of a determined number of reaction sites simultaneously while maintaining a temperature of the determined number of reaction sites at a desired temperature; and
collecting multiple images of the device, wherein collecting multiple images comprises;
collecting at least a first image at a first wavelength; and
collecting at least a second image at a second wavelength different than the first wavelength.
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Abstract
An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.
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Citations
53 Claims
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1. A method of imaging electromagnetic radiation emitted from a device having a plurality of reaction sites, the method comprising:
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thermally cycling the plurality of reaction sites of the device with a thermal controller; illuminating the device with electromagnetic radiation with an illumination system; transmitting the electromagnetic radiation along an optical path and through an optical filter device and a multi-element optical system coupled to the optical path, wherein the multi-element optical system comprises at least five optical elements; reducing chromatic aberration of light along the optical path; capturing an image of a determined number of reaction sites simultaneously while maintaining a temperature of the determined number of reaction sites at a desired temperature; and collecting multiple images of the device, wherein collecting multiple images comprises; collecting at least a first image at a first wavelength; and collecting at least a second image at a second wavelength different than the first wavelength. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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26. A method of imaging electromagnetic radiation emitted from a device having a plurality of reaction sites, the method comprising:
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thermally cycling greater than 383 reaction sites of the device with a thermal controller; illuminating the greater than 383 reaction sites of the device with electromagnetic radiation with an illumination system; transmitting the electromagnetic radiation along an optical path and through an optical filter device and a multi-element optical system coupled to the optical path; reducing chromatic aberration of light along the optical path; capturing a first fluorescence image of the greater than 383 reaction sites of the device while maintaining a temperature of the greater than 383 reaction sites at a desired temperature, wherein the first image is at a first wavelength; repeating the thermally cycling of the greater than 383 reaction sites; and capturing a second fluorescence image of the greater than 383 reaction sites of the device while maintaining the desired temperature, wherein the second image is at a second wavelength different than the first wavelength. - View Dependent Claims (27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
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38. An optical system for imaging a plurality of indications from a plurality of reaction sites of a device, the system comprising:
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a thermal cycler thermally coupled to the plurality of reactions sites of the device, the thermal cycler configured to provide temperature control to the plurality of reaction sites; an illumination system coupled to the device and configured to illuminate the device with electromagnetic radiation; an optical lens system coupled to an optical path, the optical lens system being configured to transmit the electromagnetic radiation along the optical path, wherein the optical lens system comprises a first multi-element optical train coupled to a first portion of the optical path and a second multi-element optical train coupled to a second portion of the optical path, and wherein the optical lens system is configured to reduce chromatic aberration of light along the optical path; and an optical detection system device having a detector for capturing at least first and second images of a determined number of reaction sites simultaneously while the thermal cycler maintains the determined number of reaction sites at a constant temperature, wherein the first image is at a first wavelength and the second image is at a second wavelength different than the first wavelength. - View Dependent Claims (39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53)
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Specification