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Optical lens system and method for microfluidic devices

DC
  • US 8,926,905 B2
  • Filed: 07/09/2013
  • Issued: 01/06/2015
  • Est. Priority Date: 06/07/2004
  • Status: Active Grant
First Claim
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1. A method of imaging electromagnetic radiation emitted from a device having a plurality of reaction sites, the method comprising:

  • thermally cycling the plurality of reaction sites of the device with a thermal controller;

    illuminating the device with electromagnetic radiation with an illumination system;

    transmitting the electromagnetic radiation along an optical path and through an optical filter device and a multi-element optical system coupled to the optical path, wherein the multi-element optical system comprises at least five optical elements;

    reducing chromatic aberration of light along the optical path;

    capturing an image of a determined number of reaction sites simultaneously while maintaining a temperature of the determined number of reaction sites at a desired temperature; and

    collecting multiple images of the device, wherein collecting multiple images comprises;

    collecting at least a first image at a first wavelength; and

    collecting at least a second image at a second wavelength different than the first wavelength.

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