Heated reaction chamber for processing a biochip and method for controlling said reaction chamber

• US 8,926,922 B2
• Filed: 12/28/2011
• Issued: 01/06/2015
• Est. Priority Date: 06/27/2006
• Status: Expired due to Fees

• Current Assignee: Zentaris GmbH (Aeterna Zentaris Inc.)
• Original Assignee: Zentaris GmbH (Aeterna Zentaris Inc.)
• Inventors: Lepschi, Jana, Gohring, Jens, Heydenhaus, Stefan, Ullrich, Manuel
• Primary Examiner(s): SIEFKE, SAMUEL P
• Application Number:
  US13/338,627
• Publication Number:
  US 20120094393A1
• Time in Patent Office: 1,105 Days
• Field of Search: 422/400, 422/401, 422/430, 422/82.12
• US Class Current: 422/430
• CPC Class Codes: B01L 2200/147 Employing temperature sensors
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0822 Slides
• CPC Class Codes: B01L 2300/0851 Bottom walls
• CPC Class Codes: B01L 2300/123 Flexible; Elastomeric
• CPC Class Codes: B01L 2300/1805 Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
• CPC Class Codes: B01L 2300/1822 using Peltier elements
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2300/1894 Cooling means; Cryo cooling
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: B01L 7/5255 by moving sample containers

Abstract

The invention relates to a heated reaction chamber for processing a biochip and to a method for controlling said reaction chamber. The heated reaction chamber for processing a biochip comprises a chamber wall, constituted by a flexible circuit board (10), a circuit path (10.3) being configured on the flexible circuit board (10) and being used as the heating device. The use of a flexible circuit board as the wall of a reaction chamber allows for a low thermal capacity of the reaction chamber in the area of the heating device, thereby allowing the chamber to be heated up quickly.

Claims

1. A heated reaction chamber for processing a biochip, wherein the reaction chamber is defined at least in part by a detection window spaced apart from the biochip and a chamber wall represented by a printed circuit board on which the biochip is mounted, wherein a single heating conductor that serves as a heating device and also serves as a temperature measuring device is formed on the printed circuit board, wherein the single heating conductor is connected to a measuring and control unit capable of controlling the single heating conductor both for heating and for measuring the temperature, and wherein at least one of the printed circuit board and the detection window is sufficiently elastic to allow the biochip to be pressed into contact with the detection window.

2. The heated reaction chamber according to claim 1, wherein the measuring and control unit is capable of simultaneously measuring the temperature and heating the heating conductor.

3. The heated reaction chamber according to claim 1, wherein the measuring and control unit comprises two substantially identical measuring channels capable of measuring heating voltage and heating current.

4. The heated reaction chamber according to claim 2, wherein the measuring and control unit comprises two substantially identical measuring channels capable of measuring heating voltage and heating current.

5. The heated reaction chamber according to claim 4, wherein each of the two measuring channels is provided with an A/D converter that form a part of a synchronous two-channel A/D converter.

6. The heated reaction chamber according to claim 1, wherein the measuring and control unit is connected to pick off voltage at the heating conductor and voltage at a current measuring resistor connected in series with the heating conductor.

7. The heated reaction chamber according to claim 1, wherein the measuring and control unit is capable of scanning the temperature with a scanning rate of at least 1000 Hz.

8. The heated reaction chamber according to claim 5, wherein the measuring and control unit is capable of scanning the temperature with a scanning rate of at least 1000 Hz.

9. The heated reaction chamber according to claim 1, wherein the heating conductor is located on a side of the printed circuit board which faces away from the reaction chamber, and wherein a temperature homogenization layer of a thermally conductive metal material is provided on a side of the printed circuit board which faces inwards towards the reaction chamber.

10. The heated reaction chamber according to claim 8, wherein the heating conductor is located on a side of the printed circuit board which faces away from the reaction chamber, and wherein a temperature homogenization layer of a thermally conductive metal material is provided on a side of the printed circuit board which faces inwards towards the reaction chamber.

11. The heated reaction chamber according to claim 1, wherein the heating conductor has a shape selected from the group consisting of a meandering shape or a double spiral.

12. The heated reaction chamber according to claim 1, wherein the heating conductor has a resistance of approximately 5 to 100 ohms at room temperature.

13. The heated reaction chamber according to claim 10, wherein the heating conductor has a resistance of approximately 5 to 100 ohms at room temperature.

14. The heated reaction chamber according to claim 1, wherein the heating conductor is made of copper.

15. The heated reaction chamber according to claim 1, wherein a semiconductor memory for storing data specific to the reaction chamber is provided on the printed circuit board and is connected to the control unit for controlling the heating and measuring current via conductors.

16. The heated reaction chamber according to claim 1, wherein the biochip is mounted on the printed circuit board in a region of the heating conductor.

17. The heated reaction chamber according to claim 16, wherein the heating conductor extends across a region which is larger than the biochip.

18. The heated reaction chamber according to claim 1, wherein the reaction chamber is a part of a cartridge containing a equalization chamber communicating with the reaction chamber via a equalization passage.

19. The heated reaction chamber according to claim 18, wherein a window is provided in the equalization passage.

20. The heated reaction chamber according to claim 18, wherein the cartridge is provided with a filling port wherein a check valve is located, and wherein the filling port communicates with the reaction chamber through a filling passage.

21. The heated reaction chamber according to claim 20, wherein a self-contained communicating fluid passage is provided between the filling port and the equalization chamber.

22. The heated reaction chamber according to claim 1, wherein a cooling device equipped with a cooling piston is provided, which can be brought into contact with the reaction chamber in order to cool it.

23. The heated reaction chamber according to claim 21, wherein a cooling device equipped with a cooling piston is provided, which can be brought into contact with the reaction chamber in order to cool it.

24. The heated reaction chamber according to claim 22, wherein the cooling device comprises a drive for the automatic movement of the cooling piston, enabling the latter to contact the printed circuit board with a cooling surface.

25. A method for controlling a heated reaction chamber for processing a biochip, wherein the reaction chamber is defined at least in part by a detection window spaced apart from the biochip and a chamber wall represented by a printed circuit board on which the biochip is mounted, wherein a conductor serving as a heating device is formed on the printed circuit board, wherein a current for simultaneous heating and temperature measurement is made to flow through the heating conductor in a heating phase, and wherein at least one of the printed circuit board and the detection window is sufficiently elastic to allow the biochip to be pressed into contact with the detection window.

26. The method according to claim 25, wherein the temperature is measured with a scanning rate of at least 1000 Hz.

27. The method according to claim 25, wherein a proportional-integral controller is used within a preset temperature interval about a set temperature and a proportional controller is used outside said preset temperature interval.

28. The method according to claim 25, wherein a control variable is determined from the difference between a set temperature and an actual temperature, and wherein, if the control variable is less than a preset minimum, the cooling piston is pressed against the reaction chamber.

29. The method according to claim 28, wherein if the control variable is less than zero and more than the preset minimum, the cooling piston is set at a distance from the reaction chamber.

30. The method according to claim 28, wherein the reaction chamber is heated if the control variable is more than zero.