Antibodies to the E1 extracellular loop of ion channels

• US 8,926,977 B2
• Filed: 10/27/2010
• Issued: 01/06/2015
• Est. Priority Date: 10/27/2009
• Status: Active Grant

• Current Assignee: Ucb Biopharma Sprl (UCB SA)
• Original Assignee: UCB Pharma SA (UCB SA)
• Inventors: Miller, Karen Margrete, De Ryck, Marc Roger, Wolff, Christian Gilbert J., Lawson, Alastair David Griffiths, Finney, Helene Margaret, Baker, Terence Seward
• Primary Examiner(s): Saoud, Christine J
• Assistant Examiner(s): LOCKARD, JON MCCLELLAND
• Application Number:
  US13/504,234
• Publication Number:
  US 20120259096A1
• Time in Patent Office: 1,532 Days
• Field of Search: None
• US Class Current: 424/143.1
• CPC Class Codes: C07K 16/18 against material from animals or humans
• CPC Class Codes: C07K 16/28 against receptors, cell surface antigens or cell surface determinants
• CPC Class Codes: C07K 2317/34 Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues

Abstract

An anti-E1 ion channel antibody or binding fragment thereof, pharmaceutical compositions comprising said antibodies, use of the antibodies and compositions comprising the same, in treatment, for example in the treatment/modulation of pain and processes for generating and preparing said antibodies.

Claims

1. An isolated anti-ion channel antibody or binding fragment thereof, which binds to an E1 extracellular loop of the ion channel, wherein said ion channel has a function in the modulation of pain, and said antibody or fragment is functionally modifying to said ion channel after binding thereto, wherein the ion channel is a sodium ion channel selected from the group consisting of Na_v 1.3, 1.6, 1.7, 1.8 and 1.9.

2. An anti-ion channel antibody or binding fragment thereof according to claim 1, wherein the antibody binds the E1 loop in domain A, domain B, domain C or domain D of the ion channel.

3. An anti-ion channel antibody or binding fragment thereof according to claim 1 wherein the antibody or fragment is an anti-Na_v1.7 antibody or fragment.

4. An anti-ion channel antibody or binding fragment thereof according to claim 3, wherein the antibody or fragment thereof binds to an amino acid sequence selected from the group consisting of SEQ ID NOs:

107 to 130.

5. An anti-ion channel antibody or binding fragment thereof according to claim 1, wherein the antibody or fragment thereof binds to a Na_v1.3 peptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:

131 to 134.

6. An anti-ion channel antibody or binding fragment thereof according to claim 1, wherein the antibody or fragment thereof binds to a Na_v1.6 peptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:

135 to 138.

7. An anti-ion channel antibody or binding fragment thereof according to claim 1, wherein the antibody or fragment thereof binds to a Na_v1.8 peptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:

139 to 145.

8. An anti-ion channel antibody or binding fragment thereof according to claim 1, wherein the antibody or fragment thereof binds to a Na_v1.9 peptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:

146 to 149.

9. An antibody or fragment according to any one of claim 1 or 2-8, wherein the antibody inhibits the function of the relevant ion channel to which it is specific in an in vitro patch clamp assay by at least 5 percent.