Differentiation of human embryonic and induced pluripotent stem cells
First Claim
Patent Images
1. An isolated population of chondrogenic cells, wherein at least 85% of said cells are at a single defined stage of chondrogenic differentiation, and wherein the isolated population of chondrogenic cells exhibits an increased level of Sox9 and Brachyury expression compared to a population of fully differentiated chondrocytes and exhibits a reduced level of aggrecan expression compared to a population of fully differentiated chondrocytes.
1 Assignment
0 Petitions
Accused Products
Abstract
The invention relates to culture systems, methods, and conditions that allow pluripotent undifferentiated hESCs or iPSCs to progressively and uniformly differentiate into cells of the chondrogenic lineage.
8 Citations
10 Claims
- 1. An isolated population of chondrogenic cells, wherein at least 85% of said cells are at a single defined stage of chondrogenic differentiation, and wherein the isolated population of chondrogenic cells exhibits an increased level of Sox9 and Brachyury expression compared to a population of fully differentiated chondrocytes and exhibits a reduced level of aggrecan expression compared to a population of fully differentiated chondrocytes.
Specification