Catalytic domains from lysyl oxidase and LOXL2

• US 8,927,700 B2
• Filed: 02/25/2013
• Issued: 01/06/2015
• Est. Priority Date: 08/21/2009
• Status: Active Grant

• Current Assignee: Gilead Biologics, Inc. (Gilead Sciences Inc.)
• Original Assignee: Gilead Biologics, Inc. (Gilead Sciences Inc.)
• Inventors: McCauley, Scott, Smith, Victoria
• Primary Examiner(s): Saidha, Tekchand
• Assistant Examiner(s): Meah, Md. Younus
• Application Number:
  US13/776,578
• Publication Number:
  US 20130157361A1
• Time in Patent Office: 680 Days
• Field of Search: 536/23.2, 435/321.2, 435/255.2
• US Class Current: 536/23.2
• CPC Class Codes: C12N 9/0022 with oxygen as acceptor (1.4.3)
• CPC Class Codes: C12Y 104/03013 Protein-lysine 6-oxidase (1.4.3.13), i.e. lysyl-oxidase

Abstract

Disclosed herein are amino acid sequences, and encoding nucleotide sequences, of isolated catalytic domains of the LOX and LOXL2 proteins from human and mouse. Methods for the preparation and use of these isolated catalytic domains are also provided.

Claims

1. An isolated polynucleotide, comprising:

a nucleotide sequence encoding a polypeptide comprising a catalytic domain of a human LOX protein, wherein;

the said polynucleotide is a cDNA and wherein the polypeptide comprises the amino acid sequence selected from the group consisting of;

SEQ ID NO;

1 and SEQ ID NO;

1 without the initial GL residues, and the polypeptide does not contain sequences from the human LOX protein outside its catalytic domain.

2. The isolated polynucleotide of claim 1, wherein the polynucleotide encoding the polypeptide does not contain introns.

3. The isolated polynucleotide of claim 1, wherein the polypeptide comprises the amino acid sequence set forth in SEQ ID NO:

1.

4. The isolated polynucleotide of claim 1, wherein the polypeptide comprises the amino acid sequence set forth in SEQ ID NO:

1 without the initial GL residues.

5. The isolated polynucleotide of claim 1, wherein the polypeptide further comprising a signal sequence, an epitope tag and a purification tag.

6. The isolated polynucleotide of claim 5, wherein the signal sequence is an immunoglobulin kappa signal sequence.

7. The isolated polynucleotide of claim 5, wherein the epitope tag is myc tag.

8. The isolated polynucleotide of claim 5, wherein the purification tag is a His6 tag.

9. An expression vector comprising the isolated polynucleotide of claim 1.

10. A recombinant host cell comprising the expression vector of claim 9.