Magnetic and/or electric label assisted detection system and method

• US 8,936,932 B2
• Filed: 10/05/2007
• Issued: 01/20/2015
• Est. Priority Date: 10/12/2006
• Status: Active Grant

• Current Assignee: Siemens Healthcare Nederland B.V. (Siemens AG)
• Original Assignee: Koninklijke Philips N.V.
• Inventors: Dittmer, Wendy Uyen, Prins, Menno Willem Jose
• Primary Examiner(s): Chin, Chris L
• Assistant Examiner(s): MOERSCHELL, RICHARD P
• Application Number:
  US12/444,698
• Publication Number:
  US 20100045267A1
• Time in Patent Office: 2,664 Days
• Field of Search: 324/204
• US Class Current: 435/288.5
• CPC Class Codes: G01N 27/745 for detecting magnetic beads used in biochemical assays concerning the assays G01N33/54326; sensors therefor G01R33/1269; automatic analysers therefor G01N35/0098
• CPC Class Codes: G01N 33/54326 Magnetic particles
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C

Abstract

A detection system (100) is described for detecting analytes in a fluid sample. The detection system (100) comprises a transporting means (6) for transporting magnetic and/or electric labels (5) after interaction between the sample fluid and the reagents towards a detection receptacle (1). The detection receptacle (1) is initially substantially magnetic and/or electric label (5) free. By transporting the magnetic and/or electric labels (5) after reaction, interference between unreacted reagents and magnetic and/or electric label-assisted detection can be reduced.

Claims

1. A detection system prepared for detecting at least one target molecule, the detection system comprising:

a first receptacle comprising a detection surface, the at least a first receptacle being substantially free of magnetic and/or electric labels prior to use;

a second receptacle comprising, prior to use, at least one primary magnetic and/or electric label for enabling magnetic and/or electric label assisted detection, the at least one primary magnetic and/or electric label being adapted for interacting with the at least one target molecule;

a first magnetic and/or electric field generator for applying a first magnetic field having a first orientation for transporting the at least one primary magnetic and/or electric label from the second receptacle to the detector surface of the first receptacle, thereby enabling detection of a primary magnetic and/or electric label assisted detection signal;

a third receptacle connected to an interconnection channel between the first and second receptacle and comprising at least one secondary magnetic and/or electric label being adapted for interacting with the at least one primary magnetic and/or electric label;

a second magnetic and/or electric field generator for applying a second magnetic field having a second orientation for transporting the at least one secondary magnetic and/or electric label from the third receptacle to the detector surface of the first receptacle, thereby enabling detection of a secondary magnetic and/or electric label assisted detection signal.

2. A detection system according to claim 1, wherein said magnetic and/or electric label is a magnetic label.

3. A detection system according to claim 1, wherein the detection system is adapted for enabling a fluid flow from the first receptacle to the second receptacle.

4. A detection system according to claim 3, wherein the detection system comprises at least a first channel for connecting the first receptacle and the second receptacle, the first channel being adapted for enabling said fluid flow from the first receptacle to the second receptacle by capillary action.

5. A detection system according to claim 1,

wherein the detection surface includes different locations for detection of different targets.

6. A detection system according to claim 1, wherein the first receptacle is adapted for allowing the interaction between the at least one target molecule and the at least one magnetic and/or electric label.

7. A detection system according to claim 1, wherein the second receptacle is adapted for allowing interaction between a sample, an assay reagent and the magnetic and/or electric labels.

8. A detection system according to claim 1, wherein the first receptacle and/or the second receptacle are in contact with fluid flow rate modifying moieties.

9. The detection system of claim 8, wherein the fluid flow rate modifying moieties each comprise an absorbent material each connected to one of the first and second receptacles via a corresponding channel.

10. The detection system of claim 9, further comprising valves disposed at openings of the first and second receptacles between the first and second receptacles and the fluid flow rate modifying moieties.

11. A detection system according to claim 1, the detection system comprising a fluid detector in the first receptacle and/or the second receptacle.

12. The detection system of claim 11, wherein the fluid detector detects a time of arrival at the first receptacle of a fluid containing the at least one target molecule.

13. The detection system of claim 1, further comprising a fluid flow control device for causing a fluid flow of reagents from the first receptacle to the second receptacle.

14. The detection system of claim 13, wherein the fluid flow control device comprises a pump.

15. The detection system of claim 1, wherein the first and second orientations are substantially orthogonal relative to each other.

16. The detection system of claim 1, wherein the first magnetic field transports the at least one primary magnetic and/or electric label along the first orientation without transporting the at least one secondary magnetic and/or electric label;

and

the second magnetic field transports the at least one secondary magnetic and/or electric label along the second orientation without transporting the at least one primary magnetic and/or electric label.

17. The detection system of claim 1, wherein the first and second magnetic field generators are configured to actuate the first and second magnetic fields in sequence to:

transport the at least one primary magnetic and/or electric label along the first orientation from the second receptacle to the first receptacle; and

transport the at least one secondary magnetic and/or electric label along the second orientation from the third receptacle to the second receptacle.

18. The detection system of claim 1, wherein the first magnetic field generator is configured to:

apply the first magnetic field to transport the at least one primary magnetic and/or electric label along the first orientation from the second receptacle to the detector surface of the first receptacle;

after the at least one primary magnetic and/or electric label is bound to the detector surface, apply the first magnetic field to transport at least one unbound primary magnetic and/or electric label in an opposite direction along the first orientation to the second receptacle.

19. The detection system of claim 18, wherein the second magnetic field generator is configured to:

apply the second magnetic field to transport the at least one secondary magnetic and/or electric label along the second orientation from the third receptacle to the detector surface of the first receptacle.

20. A sensor component prepared for use with a detection system for detecting at least one target molecule, the sensor component comprising:

a first receptacle comprising a detection surface, the first receptacle being substantially magnetic and/or electric label-free prior to use,

a first fluid flow rate modifying moiety comprised of an absorbent material and connected via a first channel to the first receptacle,

a first valve at an opening of the first receptacle for controlling a timing of a flow of a sample between the first receptacle and the first fluid flow rate modifying moiety,

a second receptacle comprising, prior to use, at least one magnetic and/or electric label for enabling magnetic and/or electric label assisted detection, the at least one magnetic and/or electric label adapted for interacting with the at least one target molecule,

a second fluid flow rate modifying moiety comprised of an absorbent material and connected via a second channel to the second receptacle,

a second valve at an opening of the second receptacle for controlling a timing of a flow of the sample between the second receptacle and the second fluid flow rate modifying moiety,

a fluid flow control device for causing a fluid flow of reagents from the first receptacle to the second receptacle,

wherein the sensor component is adapted for enabling transport of the at least one magnetic and/or electric label from the second receptacle to the first receptacle using a magnetic and/or electric field.

21. The sensor component of claim 20, wherein the fluid flow control device comprises a pump.

22. The sensor component of claim 20, further comprising a channel connected to the second receptacle for directing away from the first receptacle a sample received at the second receptacle from the first receptacle.

23. A sensor component for detecting at least one target molecule, the sensor component comprising:

a first receptacle comprising a detection surface, the first receptacle being substantially magnetic and/or electric label-free prior to use;

a first fluid flow rate modifying moiety comprised of an absorbent material and connected via a first channel to the first receptacle;

a first valve at an opening of the first receptacle for controlling a timing of a flow of a sample between the first receptacle and the first fluid flow rate modifying moiety;

a second receptacle comprising, prior to use, at least one magnetic and/or electric label for enabling magnetic and/or electric label assisted detection;

a second fluid flow rate modifying moiety comprised of an absorbent material and connected via a second channel to the second receptacle;

a second valve at an opening of the second receptacle for controlling a timing of a flow of the sample between the second receptacle and the second fluid flow rate modifying moiety; and

a fluid flow control device for causing a fluid flow of reagents from the first receptacle to the second receptacle.