Test sensor reagent having cellulose polymers
First Claim
1. A test sensor reagent composition adapted to assist in determining an analyte concentration of a fluid sample, the reagent comprising an enzyme, an electron transfer mediator, a cellulose polymer and a rheological additive, wherein the rheological additive is smectite clay, wherein the test sensor reagent composition comprises from about 3.6 wt. % to about 6.0 wt. % of a hydroxyethyl cellulose polymer, from about 1 wt. % to about 4 wt. % of a glucose oxidase enzyme, from about 15 wt. % to about 20 wt. % of a ferricyanide mediator and from about 0.2 wt. % to about 1.6 wt. % of the smectite clay.
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Abstract
A test sensor reagent for measuring the concentration of analytes in body fluids includes cellulose polymers for improving the stability of the test sensor and reducing the total assay time. The test sensor reagent also includes an enzyme, an electron transfer mediator and a rheological additive.
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Citations
15 Claims
- 1. A test sensor reagent composition adapted to assist in determining an analyte concentration of a fluid sample, the reagent comprising an enzyme, an electron transfer mediator, a cellulose polymer and a rheological additive, wherein the rheological additive is smectite clay, wherein the test sensor reagent composition comprises from about 3.6 wt. % to about 6.0 wt. % of a hydroxyethyl cellulose polymer, from about 1 wt. % to about 4 wt. % of a glucose oxidase enzyme, from about 15 wt. % to about 20 wt. % of a ferricyanide mediator and from about 0.2 wt. % to about 1.6 wt. % of the smectite clay.
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4. A method of determining an analyte concentration of a fluid sample comprising the acts of:
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providing an electrochemical test sensor adapted to assist in determining the analyte concentration, the electrochemical test sensor comprising a plurality of electrodes including a first electrode and a second electrode, a fluid receiving area and a test sensor reagent, the test sensor reagent composition comprising a cellulose polymer, a rheological additive, and an enzyme, the rheological additive is smectite clay, wherein the test sensor reagent composition comprises from about 3.6 wt. % to about 6.0 wt. % of a hydroxyethyl cellulose polymer, from about 1 wt. % to about 4 wt. % of a glucose oxidase enzyme, from about 15 wt. % to about 20 wt. % of a ferricyanide mediator and from about 0.2 wt. % to about 1.6 wt. % of the smectite clay; and determining the analyte concentration in an assay time of less than about 35 seconds. - View Dependent Claims (5, 6)
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7. A method of determining an analyte concentration of a fluid sample, the method comprising the acts of:
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providing a fluid sample; contacting a test sensor with the fluid sample, the fluid sample having at least one analyte; contacting the fluid sample with a reagent composition comprising a cellulose polymer, a rheological additive and an enzyme, the rheological additive is smectite clay, wherein the test sensor reagent composition comprises from about 3.6 wt. % to about 6.0 wt. % of a hydroxyethyl cellulose polymer, from about 1 wt. % to about 4 wt. % of a glucose oxidase enzyme, from about 15 wt. % to about 20 wt. % of a ferricyanide mediator and from about 0.2 wt. % to about 1.6 wt. % of the smectite clay; providing an electrical signal indicative of the analyte in the fluid sample; and determining the analyte using the electrical signal. - View Dependent Claims (8)
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9. A method of determining an analyte concentration of a fluid sample, the method comprising the acts of:
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providing the fluid sample; contacting a test sensor with the fluid sample, the fluid sample having at least one analyte; contacting the fluid sample with a reagent composition comprising a cellulose polymer, a rheological additive and an enzyme, the rheological additive is smectite clay, wherein the test sensor reagent composition comprises from about 1 wt. % to about 10 wt. % of a hydroxyethyl cellulose polymer, from about 0.5 wt. % to about 5 wt. % of a glucose oxidase enzyme, from about 1 wt. % to about 20 wt. % of a ferricyanide mediator and from about 0.2 wt. % to about 3 wt. % of the smectite clay; and determining the analyte concentration of the fluid sample. - View Dependent Claims (10)
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- 11. A test sensor reagent composition adapted to assist in determining an analyte concentration of a fluid sample, the reagent comprising an enzyme, an electron transfer mediator, a cellulose polymer and a rheological additive, wherein the cellulose polymer, the rheological additive and the enzyme are present in a single layer, and wherein the rheological additive is a smectite clay, wherein the test sensor reagent composition comprises from about 1 wt. % to about 10 wt. % of a hydroxyethyl cellulose polymer, from about 0.5 wt. % to about 5 wt. % of a glucose oxidase enzyme, from about 1 wt. % to about 20 wt. % of a ferricyanide mediator and from about 0.2 wt. % to about 3 wt. % of the smectite clay, wherein the hydroxyethyl cellulose polymer has a molecular weight from about 25,000 to about 2,000,000.
- 13. A test sensor reagent composition adapted to assist in determining an analyte concentration of a fluid sample, the reagent comprising an enzyme, an electron transfer mediator, a cellulose polymer and a rheological additive, wherein the test sensor reagent composition comprises from about 1 wt. % to about 10 wt. % of a hydroxyethyl cellulose polymer, from about 0.5 wt. % to about 5 wt. % of a glucose oxidase enzyme, from about 1 wt. % to about 20 wt. % of a ferricyanide mediator and from about 0.2 wt. % to about 3 wt. % of the rheological additive, wherein the hydroxyethyl cellulose polymer has a molecular weight from about 25,000 to about 2,000,000.
Specification