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Methods of isolating and culturing stem cells

  • US 8,940,294 B2
  • Filed: 03/01/2013
  • Issued: 01/27/2015
  • Est. Priority Date: 03/02/2012
  • Status: Active Grant
First Claim
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1. A method for isolating an E-cadherin positive stem cell, comprising:

  • (a)removing all epithelial cells from an isolated mixed cell population comprising one or more stem cells and one or more non-stem cells; and

    (b)after removal of all of the epithelial cells contacting the remaining cells of the mixed cell population with an agent that binds to E-cadherin, thereby isolating an E-cadherin positive stem cell from the mixed cell population.

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