Detection device and methods of use

• US 8,940,481 B2
• Filed: 02/12/2010
• Issued: 01/27/2015
• Est. Priority Date: 02/05/2007
• Status: Active Grant

• Current Assignee: QIAGEN Sciences, LLC (Qiagen NV)
• Original Assignee: Intelligent Bio-Systems Incorporated. (Qiagen NV)
• Inventors: Gordon, Steven, Auger, Steven, Schinazi, Alan
• Primary Examiner(s): Kim, Young J
• Application Number:
  US12/704,842
• Publication Number:
  US 20100152050A1
• Time in Patent Office: 1,810 Days
• Field of Search: None
• US Class Current: 435/6.1
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: G01N 2021/0325 Cells for testing reactions, e.g. containing reagents
• CPC Class Codes: G01N 2021/6421 Measuring at two or more wavelengths
• CPC Class Codes: G01N 2021/6441 with two or more labels
• CPC Class Codes: G01N 2021/6471 Special filters, filter wheel
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence

Abstract

An imaging system for exciting and measuring fluorescence on or in samples comprising fluorescent materials (e.g. fluorescent labels, dyes or pigments). In one embodiment, a device is used to detect fluorescent labels on nucleic acid. In a preferred embodiment, the device is configured such that fluorescent labels in a plurality of different DNA templates are simultaneously detected.

Claims

1. A method for carrying out process steps for nucleic acid sequencing, comprising:

a) providing a first chip in a first flow cell, a second chip in a second flow cell, nucleic acid to be sequenced, nucleic acid sequencing reagents, a first camera and a second camera;

b) introducing said nucleic acid to be sequenced and said nucleic acid sequencing reagents into said first and second flow cells under conditions such that, while said first chip in said first flow cell is undergoing one or more reaction steps, said second chip in a second flow cell is being scanned and imaged with said first camera; and

c) scanning and imaging said first chip in said first flow cell with said second camera.

2. The method of claim 1, wherein said nucleic acid sequencing reagents comprise those required to produce fluorescent signals.

3. The method of claim 1, wherein said first and second chips comprise arrays of nucleic acid spots.

4. The method of claim 3, wherein the nucleic acid sequencing reagents pass the spots and produce the signals required for sequencing.

5. The method of claim 1, wherein the second chip remains in the flow cell while it is imaged.

6. The method of claim 1, wherein said first and second flow cells are positioned on a moving support.

7. The method of claim 6, wherein said moving support is a rotary stage.

8. The method of claim 1, wherein said first and second flow cells are transparent.

9. The method of claim 1, wherein said flow cells are incorporated within an instrument.

10. A method for carrying out process steps for nucleic acid sequencing, comprising:

a) providing i) a first chip in a first flow cell, a second chip in a second flow cell, said first and second flow cells positioned on a moving support, ii) nucleic acid to be sequenced, nucleic acid sequencing reagents, and iii) first and second cameras;

b) introducing said nucleic acid to be sequenced and said nucleic acid sequencing reagents into said first and second flow cells under conditions such that, while said first chip in said first flow cell is undergoing one or more reaction steps, said second chip in a second flow cell is being scanned and imaged with said first camera; and

c) scanning and imaging said first chip in said first flow cell with said second camera after step b).

11. The method of claim 10, wherein said moving support is a rotary stage.

12. A method for carrying out process steps for nucleic acid sequencing, comprising:

a) providing i) a first array of nucleic acid in a first flow cell, a second array of nucleic acid in a second flow cell, said first and second flow cells positioned on a moving support, ii) nucleic acid sequencing reagents capable of producing fluorescent signals from fluorescent labels covalently attached to a nucleotide that can be incorporated in said nucleic acid, iii) reagents required to remove fluorescent signals, and iv) at least one camera;

b) introducing nucleic acid sequencing reagents into said second flow cell under conditions so as to generate first fluorescent signals in said second array;

c) introducing nucleic acid sequencing reagents into said first flow cells under conditions such that, while said first array in said first flow cell is undergoing one or more reaction steps so as to produce fluorescent signals, said second array in a second flow cell is being imaged with said at least one camera;

d) moving said second flow cell on said moving support to a position where the reagents required to remove the fluorescent signals in said second array are delivered to said second flow cell to create a treated second flow cell;

e) moving said first flow cell on said moving support and imaging said first array in said first flow cell with said at least one camera;

f) introducing nucleic acid sequencing reagents into said treated second flow cell under conditions so as to generate second fluorescent signals in said second array; and

g) imaging said treated second flow cell after step f) with said at least one camera.

13. The method of claim 12, wherein said moving support is a rotary stage.

14. The method of claim 12, wherein said nucleic acid is arrayed on a surface of the flow cell.

15. The method of claim 12, wherein said first and second flow cells comprise a fluid channel and said nucleic acid is arrayed on said fluid channel.