Methods for genotyping mature cotton fibers and textiles
First Claim
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1. A method for identifying cotton cultivar comprising:
- collecting cotton fibers from a manufactured textile;
extracting genomic DNA from the cotton fibers collected, wherein the genomic DNA extracted from the mature cotton fibers comprises chloroplast DNA;
amplifying said chloroplast DNA with PCR-based techniques using one or more primer sets and thereby producing one or more amplicons;
generating a variable amplicon length polymorphism profile of said chloroplast DNA;
comparing the variable amplicon length polymorphism profile for the mature cotton fibers from the manufactured textile with a known variable amplicon lengthand thereby identifying at least one cotton cultivar from the manufactured textile.
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Abstract
Methods for distinguishing between cotton cultivars of a specific species by analyzing a sample of mature cotton fibers from raw cotton materials or from textile goods are disclosed. DNA is extracted from the mature cotton fiber sample and subjected to PCR techniques which enable the identification of the cultivar of a particular cotton species utilized in the textile or cotton material of interest.
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Citations
31 Claims
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1. A method for identifying cotton cultivar comprising:
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collecting cotton fibers from a manufactured textile; extracting genomic DNA from the cotton fibers collected, wherein the genomic DNA extracted from the mature cotton fibers comprises chloroplast DNA; amplifying said chloroplast DNA with PCR-based techniques using one or more primer sets and thereby producing one or more amplicons; generating a variable amplicon length polymorphism profile of said chloroplast DNA; comparing the variable amplicon length polymorphism profile for the mature cotton fibers from the manufactured textile with a known variable amplicon length and thereby identifying at least one cotton cultivar from the manufactured textile. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 24, 25, 31)
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14. A method for verifying the authenticity of a manufactured textile comprising:
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collecting mature cotton fibers from the manufactured textile; extracting genomic DNA from the comprising chloroplast DNA from the mature cotton fibers collected; amplifying said chloroplast DNA with PCR-based techniques using one or more primer sets producing one or more amplified products forming a variable amplicon length polymorphism profile for the mature cotton fibers from the textile article; comparing the variable amplicon length polymorphism profile from the mature cotton fibers with one or more known variable amplicon length polymorphism profiles; identifying the mature cotton fibers from the manufactured textile as belonging to one or more cotton cultivars; and thereby determining whether the manufactures textile is authentic or counterfeit. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23, 26, 27, 28, 29, 30)
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Specification