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Methods for genotyping mature cotton fibers and textiles

  • US 8,940,485 B2
  • Filed: 11/12/2008
  • Issued: 01/27/2015
  • Est. Priority Date: 11/12/2008
  • Status: Active Grant
First Claim
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1. A method for identifying cotton cultivar comprising:

  • collecting cotton fibers from a manufactured textile;

    extracting genomic DNA from the cotton fibers collected, wherein the genomic DNA extracted from the mature cotton fibers comprises chloroplast DNA;

    amplifying said chloroplast DNA with PCR-based techniques using one or more primer sets and thereby producing one or more amplicons;

    generating a variable amplicon length polymorphism profile of said chloroplast DNA;

    comparing the variable amplicon length polymorphism profile for the mature cotton fibers from the manufactured textile with a known variable amplicon lengthand thereby identifying at least one cotton cultivar from the manufactured textile.

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