Optical detector for use in therapy
First Claim
1. A method of delivering a therapy using an implantable medical device, comprising:
- presenting a tagged substrate into a bodily fluid, wherein the bodily fluid is blood, wherein the bodily fluid contains an enzyme and a protein substrate, and wherein presenting the tagged substrate to the bodily fluid comprises controlling a substrate pump to present the tagged substrate to the blood via a catheter with a distal end located in a blood vessel of a patient, and further wherein the tagged substrate is tagged with at least two fluorescent dyes;
emitting energy at a first wavelength into the bodily fluid that contains the enzyme and the protein substrate, and into which the substrate tagged with the at least two fluorescent dyes has been presented;
detecting energy emitted by a first one of the dyes at a second wavelength based on fluorescent resonant energy transfer from a second one of the dyes in response to the emitted energy at the first wavelength; and
providing a therapy device comprising a therapy pump configured to deliver a therapy based on the detected energy.
1 Assignment
0 Petitions
Accused Products
Abstract
Activation of an enzyme in a bodily fluid is detected based on the amount of cleavage of a substrate for the enzyme. The substrate is tagged with two fluorescent dyes—a donor and an acceptor. The tagged substrate is presented to the bodily fluid. A device emits energy at a first wavelength into the bodily fluid, and detects energy at second and third wavelengths emitted by the dyes in response to the energy at the first wavelength. Prior to enzymatic cleavage of the substrate, the acceptor emits energy at the third wavelength in response to energy at the second wavelength received through fluorescent resonant energy transfer (FRET) from the donor. After enzymatic cleavage of the substrate, the donor emits energy at the second wavelength. The device can determine the concentration of activated enzyme within the bodily fluid based on the relative intensities of energy at the second and third wavelengths.
-
Citations
10 Claims
-
1. A method of delivering a therapy using an implantable medical device, comprising:
-
presenting a tagged substrate into a bodily fluid, wherein the bodily fluid is blood, wherein the bodily fluid contains an enzyme and a protein substrate, and wherein presenting the tagged substrate to the bodily fluid comprises controlling a substrate pump to present the tagged substrate to the blood via a catheter with a distal end located in a blood vessel of a patient, and further wherein the tagged substrate is tagged with at least two fluorescent dyes; emitting energy at a first wavelength into the bodily fluid that contains the enzyme and the protein substrate, and into which the substrate tagged with the at least two fluorescent dyes has been presented; detecting energy emitted by a first one of the dyes at a second wavelength based on fluorescent resonant energy transfer from a second one of the dyes in response to the emitted energy at the first wavelength; and providing a therapy device comprising a therapy pump configured to deliver a therapy based on the detected energy. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
-
Specification