Apparatus and method for quantifying binding and dissociation kinetics of molecular interactions

• US 8,940,538 B2
• Filed: 10/29/2010
• Issued: 01/27/2015
• Est. Priority Date: 11/23/2009
• Status: Active Grant

• Current Assignee: Korea Research Institute of Standards and Science
• Original Assignee: Korea Research Institute of Standards and Science
• Inventors: Cho, Hyun Mo, Che, Gal Won, Cho, Yong Jai
• Primary Examiner(s): Soderquist, Arlen
• Application Number:
  US13/511,330
• Publication Number:
  US 20120295357A1
• Time in Patent Office: 1,551 Days
• Field of Search: 422/82.05, 422/82.09, 422401-404, 422/408, 422/412, 422/425, 422/430, 422501-502, 422/507, 436/34, 436 85- 87, 436 94- 95, 436164-165, 436/171, 436/518, 436/524, 436/527
• US Class Current: 436/34
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2400/086 using baffles or other fixed flow obstructions
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: G01N 21/211 Ellipsometry optical thickness measurement G01B11/06
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]
• CPC Class Codes: Y10T 436/144444 Glucose

Abstract

The present invention relates to an apparatus for quantifying the binding and dissociation kinetics of molecular interactions of small molecular bio materials with high sensitivity almost without the influence of a change in the reflective index resulting from a buffer solution by making polarized incident light incident on the binding layer of a bio material, formed in a thin dielectric film, so that the polarized incident light satisfies a p-wave non-reflecting condition and a quantifying method using the same.

Claims

1. An apparatus for quantifying binding and dissociation kinetics of molecular interactions, the apparatus comprising:

a micro flow path structure comprising

a support,

a substrate on the support and having a semiconductor material,

a dielectric film on the substrate,

a cover unit on the support and having an incident window and a reflection window respectively on one side and the other side and,

said support defining micro flow paths between the support and the cover unit, the micro flow paths comprising a plurality of inflow paths, and a plurality of outflow paths on one side and the other side of the support, respectively,

the micro flow paths of multiples channels having a plurality of barrier ribs in an inner face of the cover unit and to connect the inflow paths and the outflow paths, respectively;

a sample injection unit for forming a binding layer of samples on the dielectric film by injecting a buffer solution comprising the samples of a bio material into the micro flow paths;

a polarization generation unit for radiating incident light, polarized through the incident window, to the binding layer at an angle of incidence θ

satisfying a p-wave non-reflecting condition; and

a polarization detection unit for detecting a change in a polarization of reflected light of the binding layer incident through the reflection window;

each of the incident window and the reflection window of the cover unit having a curved shell form;

the incident light and the reflected light being incident on the incident window and the reflection window, respectively, adjacent a vertical angle of the extent that a polarization state of each of the incident light and the reflected light is not greatly changed.

2. The apparatus according to claim 1, wherein the dielectric film comprises at least one of a transparent material semiconductor oxide film and a transparent material glass film.

3. The apparatus according to claim 2, wherein the dielectric film has a thickness of 0 to 1000 nm.

4. The apparatus according to claim 1, wherein the cover unit is integrally made of at least one of glass and a transparent synthetic resin material.

5. The apparatus according to claim 1, wherein the binding layer is a multi-layered film comprising self-assembled monolayers for bonding characteristics of various bio materials, a fixation material, and various bio materials including small molecules bonded to the fixation material.

6. The apparatus according to claim 1, wherein the polarization generation unit comprises a light source for radiating light, and a polarizer for polarizing the radiated light.

7. The apparatus according to claim 6, wherein the light source radiates at least one of monochromatic light and white light.

8. The apparatus according to claim 6, wherein the light source comprises at least one of a laser and a laser diode having a wavelength-variable structure.

9. The apparatus according to claim 6, wherein the polarization generation unit comprises at least one of a collimation lens for providing parallel light to the polarizer, a focusing lens for increasing an amount of the incident light by converging the parallel light passing through the polarizer, and a first compensator 350 for phase-delaying polarized components of the incident light.

10. The apparatus according to claim 9, wherein the polarizer and the first compensator are configured with other polarization modulations.

11. The apparatus according to claim 1, wherein the polarization detection unit comprises:

an analyzer configured to polarize the reflected light;

a photodetector configured to obtain optical data by detecting the polarized reflected light; and

an operation processor coupled to the photodetector and generating values based on the optical data.

12. The apparatus according to claim 11, wherein the photodetector comprises at least one of a charge-coupled device (CCD) type solid state array, a photomultiplier tube, and a silicon photodiode.

13. The apparatus according to claim 11, wherein the operation processor generates a binding concentration value, a binding constant value, and a dissociation constant value of the samples, by finding an ellipsometric constant Ψ

or Δ

.

14. The apparatus according to claim 11, wherein the polarization detection unit further comprises at least one of a second compensator for phase-delaying polarized components of the reflected light, and a spectroscope for making spectroscopic the reflected light.

15. The apparatus according to claim 14, wherein the analyzer and the second compensator are configured with other polarization modulations.

16. A method of quantifying binding and dissociation kinetics of molecular interactions, the method comprising:

using a sample injection unit for injecting a buffer solution including samples of small molecular bio materials into micro flow paths of a micro flow path structure, the buffer solution including the samples of different concentrations and being injected into respective micro flow paths of the micro flow path structure including multiple channels;

binding the samples to a dielectric film of the micro flow path structure for forming a binding layer, the samples being bound to a plurality of different self-assembled monolayers formed on the dielectric film for forming different binding layers;

using a polarization generation unit for polarizing light and making the polarized light incident on the binding layer at an angle of incidence to satisfy a p-wave non-reflecting condition through an incident window of the micro flow path structure;

using reflected light from the binding layer being incident on a polarization detection unit through a reflection window of the micro flow path structure; and

using the polarization detection unit for detecting a polarization state of the reflected light using at least one of ellipsometry and reflectometry.

17. The method according to claim 16, wherein the detecting of the polarization state comprises:

using an analyzer for polarizing the reflected light;

using a photodetector for obtaining optical data by detecting the polarized reflected light; and

using an operation processor for generating a binding value concentration, a binding constant value, and a dissociation constant value of the samples, by finding an ellipsometric constant Ψ

or Δ

of the ellipsometry on the basis of the optical data.