Reagent preparation and dispensing device and methods for the same

• US 8,940,539 B2
• Filed: 05/14/2009
• Issued: 01/27/2015
• Est. Priority Date: 05/14/2008
• Status: Active Grant

• Current Assignee: Biolyph, LLC
• Original Assignee: Biolyph, LLC
• Inventors: Pearcy, Timothy, Skakoon, James G.
• Primary Examiner(s): SIEFKE, SAMUEL P
• Application Number:
  US12/992,552
• Publication Number:
  US 20110127294A1
• Time in Patent Office: 2,084 Days
• Field of Search: 422/514, 604/82, 436/4, 436/723
• US Class Current: 436/43
• CPC Class Codes: A61J 1/06 Ampoules or carpules syringe ampoules or carpules A61M5/28
• CPC Class Codes: A61J 1/201 having one piercing end
• CPC Class Codes: A61J 1/2013 having two piercing ends
• CPC Class Codes: A61J 1/2079 Filtering means
• CPC Class Codes: A61J 1/2089 Containers or vials which are to be joined to each other in order to mix their contents combinations of vial and syringe for mixing their contents A61J1/2096
• CPC Class Codes: B01L 2200/028 Modular arrangements
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/0672 Integrated piercing tool
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 2400/049 vacuum
• CPC Class Codes: B01L 2400/0683 mechanically breaking a wall or membrane within a channel or chamber
• CPC Class Codes: B01L 3/0272 Dropper bottles
• CPC Class Codes: G01N 35/1002 Reagent dispensers
• CPC Class Codes: Y10T 137/0402 Cleaning, repairing, or assembling
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/25 including sample preparation
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A reagent preparation and dispensing device includes a body. A reagent reservoir containing a reagent is disposed within the body. A solution reservoir containing a solution is disposed within the body. The device further includes an activator movably coupled with the body to open the reagent reservoir and the solution reservoir, The activator is operable to mix the solution with the reagent to form a specified amount of a reagent mixture. A dispensing reservoir tip is coupled with the body. The dispensing reservoir tip is sized and shaped to hold the specified amount of the reagent mixture and dispense the specified amount of the reagent mixture from the device.

Claims

1. A method of making a reagent preparation and dispensing device comprising:

positioning a solution reservoir within a body, the solution reservoir including a solution therein;

lyophilizing a reagent, wherein lyophilizing of the reagent is isolated from the body and the solution reservoir, and the body and the solution reservoir are not subject to lyophilizing conditions;

coupling a reagent reservoir with the body, the reagent reservoir including the lyophilized reagent therein, the reagent reservoir separate from the body;

coupling an activator with the body, the activator operable to;

open the solution reservoir and the reagent reservoir,

mix the solution with the lyophilized reagent and form a reagent mixture, and

dispense the reagent mixture from the body.

2. The method of claim 1, wherein coupling the activator includes coupling a first and a second plunger with the body.

3. The method of claim 1, further comprising forming a flushing chamber within the body, the flushing chamber including a flushing gas, and dispensing of the reagent mixture includes forcing the flushing gas into the reagent reservoir, the flushing gas correspondingly flushes the reagent mixture from the body while the flushing gas does not mix with the reagent mixture.

4. The method of claim 3, wherein forming the flushing chamber includes forming a flushing gas vent from the flushing chamber to ambient atmosphere, and mixing of the solution with the lyophilized reagent closes the flushing gas vent and seals the flushing chamber.

5. A reagent preparation and dispensing device comprising:

a body;

a reagent reservoir containing a reagent, the reagent reservoir disposed within the body;

a solution reservoir containing a solution, the solution reservoir disposed within the body;

an activator movably coupled with the body to open the reagent reservoir and the solution reservoir, the activator movable to mix the solution with the reagent to reconstitute the reagent and form a reagent mixture;

a flushing chamber within the body, the flushing chamber includes flushing gas therein;

a dispensing tip coupled with the body; and

a plunger movably coupled with the body and coupled with the activator, the plunger is configured to force flushing gas into the reagent reservoir from the flushing chamber, and the flushing gas correspondingly forces the reagent mixture through the dispensing tip while the flushing gas does not mix with the reagent mixture.

6. The reagent preparation and dispensing device of claim 5, wherein the plunger is movable relative to the body between an unsealed condition and a sealed condition, and:

in the unsealed condition a flushing gas vent extends from the flushing chamber and out of the body allowing the flushing gas in the flushing chamber to vent to atmosphere, and

in the sealed condition the flushing gas vent is closed and movement of the activator and the plunger forces the flushing gas into the reagent reservoir.

7. The reagent preparation and dispensing device of claim 6, wherein the plunger seals and unseals the flushing chamber.

8. The reagent preparation and dispensing device of claim 6, wherein a flushing passage extends directly from the flushing chamber to the reagent reservoir.

9. The reagent preparation and dispensing device of claim 8, wherein the flushing passage is opened in the sealed condition, and the plunger is configured to force flushing gas from the flushing chamber to the reagent reservoir through the flushing passage.

10. The reagent preparation and dispensing device of claim 8, wherein the flushing passage includes at least a portion of the flushing gas vent.

11. The reagent preparation and dispensing device of claim 5, wherein the plunger includes:

a first plunger movably coupled with the body and the first plunger is coupled with the activator, the first plunger is configured to move the solution into the reagent reservoir; and

a second plunger movably coupled with the body and the second plunger is coupled with the activator, the second plunger is configured to force the flushing gas into the reagent reservoir and correspondingly force the reagent mixture through the dispensing tip.

12. The reagent preparation and dispensing device of claim 11 comprising a barrel movably coupled with the body, the barrel includes:

a barrel nozzle including the solution reservoir, and

a piercing edge sized and shaped for piercing of a reagent reservoir seal, the piercing edge near a first barrel end.

13. The reagent preparation and dispensing device of claim 12, wherein the second plunger is configured for selective sealing with the barrel, and the barrel and the second plunger form the flushing chamber.

14. The reagent preparation and dispensing device of claim 12, wherein the barrel nozzle is positioned within the reagent reservoir and decreases a reagent reservoir volume while the activator mixes the solution with the reagent.

15. The reagent preparation and dispensing device of claim 11, wherein the first plunger is engaged with the activator in a first configuration and the first plunger and the activator move together in the first configuration, and the first plunger is slidably coupled with the activator and the second plunger in a second configuration, and the activator and the second plunger move relative to the first plunger in the second configuration.

16. The reagent preparation and dispensing device of claim 5, wherein the reagent is a dried reagent.

17. The reagent preparation and dispensing device of claim 5, wherein the flushing chamber includes air as the flushing gas.

18. The reagent preparation and dispensing device of claim 5, wherein the flushing chamber has a flushing gas volume larger than a reagent reservoir volume.

19. The reagent preparation and dispensing device of claim 5, wherein the reagent reservoir includes a conical bottom in communication with the dispensing tip, and the plunger is configured to force flushing gas into the reagent reservoir with the reagent mixture positioned in the conical bottom.

20. The reagent preparation and dispensing device of claim 5, wherein the activator is configured to eliminate a solution reservoir containing the solution.

21. A method of using a reagent preparation and dispensing device comprising:

opening a reagent reservoir disposed within a body, the reagent reservoir contains a reagent;

mixing a solution with the reagent to reconstitute the reagent and form a reagent mixture; and

dispensing the reagent mixture through a dispensing tip, dispensing the reagent mixture includes;

forcing flushing gas out of a flushing chamber and into the reagent reservoir with a plunger coupled with an activator, and

forcing the reagent mixture out of the dispensing tip with the flushing gas, the flushing gas does not mix with the reagent mixture.

22. The method of claim 21 comprising venting flushing gas within the flushing chamber to atmosphere through a flushing gas vent;

and

dispensing the reagent mixture includes closing the flushing gas vent with the plunger.

23. The method of claim 21, wherein dispensing the reagent mixture includes delivering the forced flushing gas from the flushing chamber to the reagent reservoir through a flushing passage.

24. The method of claim 23, wherein dispensing the reagent mixture includes opening the flushing passage.

25. The method of claim 21, wherein dispensing the reagent mixture includes forcing flushing gas out of the flushing chamber and directly into the reagent reservoir through a flushing passage, and the flushing chamber includes a flushing gas volume greater than a reagent reservoir volume of the reagent reservoir.

26. The method of claim 21, wherein dispensing the reagent mixture includes forcing flushing gas into the reagent reservoir with the reagent mixture positioned between the flushing gas and the dispensing tip.

27. The method of claim 21 comprising shrinking the reagent reservoir before mixing the solution with the reagent.

28. The method of claim 27, wherein shrinking the reagent reservoir includes moving a barrel nozzle movably coupled with the body into the reagent reservoir to fill a portion of a reagent reservoir volume.

29. The method of claim 21, wherein the plunger includes a first plunger coupled with the activator and a second plunger coupled with the activator, the first and second plungers are movably coupled with the body;

mixing the solution with the reagent includes moving the first plunger and the activator together; and

dispensing the reagent mixture includes moving the second plunger with the activator relative to the first plunger.

30. The method of claim 29, wherein dispensing the reagent mixture includes opening a flushing passage between the flushing chamber and the reagent reservoir, and opening the flushing passage includes disengaging a portion of the first plunger from a barrel coupled with the body.

31. The method of claim 29, wherein dispensing the reagent mixture includes engaging the second plunger with a barrel coupled with the body, and engaging the second plunger with the barrel closes the flushing chamber.

32. The method of claim 21, wherein opening the reagent reservoir includes piercing a piercing edge of a barrel through a reagent reservoir seal, and the barrel is movably coupled with the body.

33. The method of claim 21, wherein mixing the solution with the reagent includes eliminating a solution reservoir containing the solution.