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Method of determining hypoxia

  • US 8,945,824 B2
  • Filed: 04/11/2008
  • Issued: 02/03/2015
  • Est. Priority Date: 10/13/2006
  • Status: Active Grant
First Claim
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1. A method of assessing hypoxia in a gastrointestinal tract of a mammal, comprising:

  • a. collecting a blood sample from the gastrointestinal tract, said blood sample comprising plasma and blood cells;

    b. separating the plasma from the blood cells;

    c. determining the amount of lactate dehydrogenase (LDH) in the plasma and comparing the amount of lactate dehydrogenase with lactate dehydrogenase in a control sample;

    d. assessing the presence of hypoxia at the gastrointestinal tract from the amount of LDH in the plasma; and

    e. identifying a location of anastomosis based on the assessment of hypoxia at the gastrointestinal tract.

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