Thermal cycling by positioning relative to fixed-temperature heat source
First Claim
1. A thermal cycling system for performing a biological reaction at two or more different temperatures, the thermal cycling system comprising:
- a) a heat source for setting at a fixed temperature;
b) a metal sleeve capable of receiving a reaction vessel containing material upon which the biological reaction is to be performed, wherein the sleeve includes a temperature-sensing means for sensing the temperature of the sleeve; and
c) moving means operatively associated with the sleeve for altering the relative position of the heat source and the sleeve based on the temperature of the sleeve sensed by the temperature-sensing means, the thermal cycling system arranged and configured such that,i) in a first configuration the relative positions of the sleeve and the heat source, with respect to each other, are such that the sleeve achieves and maintains a first temperature; and
ii) in a second configuration the relative positions of the sleeve and the heat source, with respect to each other, are adjusted such that the sleeve achieves and maintains a second temperature.
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Accused Products
Abstract
The thermal cycling system for performing a biological reaction at two or more different temperatures comprises: a) a heat source for setting at a fixed temperature; b) a reaction vessel containing material upon which the biological reaction is to be performed; c) mechanically-operable structure for altering the relative position of the heat source and the reaction vessel so that reaction vessel first achieves and maintains a desired first temperature in the reaction vessel for starting the carrying out of the biological reaction, and then for altering the relative position of the heat source and the reaction vessel so that the reaction vessel then achieves and maintains a second temperature for continuing the carrying out of the biological reaction on the biological material, and d) temperature-sensing structure operatively associated with the reaction vessel for controlling the altering of the relative position of the heat source and the reaction vessel so that the reaction vessel achieves and maintains the desired second temperature in the reaction vessel.
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Citations
34 Claims
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1. A thermal cycling system for performing a biological reaction at two or more different temperatures, the thermal cycling system comprising:
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a) a heat source for setting at a fixed temperature; b) a metal sleeve capable of receiving a reaction vessel containing material upon which the biological reaction is to be performed, wherein the sleeve includes a temperature-sensing means for sensing the temperature of the sleeve; and c) moving means operatively associated with the sleeve for altering the relative position of the heat source and the sleeve based on the temperature of the sleeve sensed by the temperature-sensing means, the thermal cycling system arranged and configured such that, i) in a first configuration the relative positions of the sleeve and the heat source, with respect to each other, are such that the sleeve achieves and maintains a first temperature; and ii) in a second configuration the relative positions of the sleeve and the heat source, with respect to each other, are adjusted such that the sleeve achieves and maintains a second temperature. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 33)
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2. A thermal cycling system for performing a polymerase chain reaction amplification protocol comprising multiple cycles of three temperature-dependent stages of template denaturation, primer annealing and primer extension that constitute a single cycle of PCR, the thermal cycling system comprising:
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a) a heat source that is set at a fixed temperature; b) a metal sleeve capable of receiving a reaction vessel containing material upon which a polymerase chain reaction amplification protocol is to be performed, wherein the sleeve includes a temperature-sensing means for sensing the temperature of the sleeve; and c) moving means operatively associated with the sleeve for altering the relative position of the heat source and the sleeve based on the temperature of the sleeve sensed by the temperature-sensing means, the thermal cycling system arranged and configured such that, i) in a first configuration the relative positions of the sleeve and the heat source, with respect to each other, are such that the sleeve achieves and maintains a first temperature for carrying out template denaturation on the material; ii) in a second configuration the relative positions of the sleeve and the heat source, with respect to each other, are adjusted such that the sleeve achieves and maintains a second temperature for carrying out primer annealing on the material; and iii) in a third configuration the relative positions of the sleeve and the heat source, with respect to each other, are adjusted such that the sleeve achieves and maintains a third temperature for carrying out primer extension on the material. - View Dependent Claims (11, 26, 27, 28, 29, 30, 31, 32, 34)
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Specification